| Title: | Protein transport in the permeabilized cell of Schizosaccharomyces pombe |
| Author(s): | Kambe-Honjoh H; Yoda K; Yamasaki M; |
| Address: | "Department of Agricultural Chemistry, University of Tokyo, Japan" |
| Journal Title: | Biochem Biophys Res Commun |
| DOI: | 10.1016/0006-291x(92)90822-3 |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "We reconstituted a protein translocation-transport system composed of permeabilized spheroplasts (P-cells) of the fission yeast Schizosaccharomyces pombe and the precursor of alpha sex pheromone, prepro-alpha-factor of the budding yeast Saccharomyces cerevisiae. We found that P-cells prepared from the spheroplasts formed in 0.7M KCl as an osmotic stabilizer had the activity to transport pro-alpha-factor to the Golgi apparatus. Electron microscopic observations showed that membranes were preserved more intact in the P-cells prepared from the spheroplasts formed in 0.7M KCl than in 0.7M sorbitol. A glycoprotein of S. pombe contains galactose residues, and we detected incorporation of radiolabeled galactose residues into the anti-prepro-alpha-factor immunoprecipitable fractions in this S. pombe system, but not in the S. cerevisiae system. This paper reports that a heterologous system of in vitro protein transport was performed, and prepro-alpha-factor has the signals necessary for early steps of the transport in S. pombe" |
| Keywords: | "Biological Transport Cell Membrane Permeability Cytosol/metabolism Fungal Proteins/genetics/*metabolism Methionine/metabolism Microscopy, Electron Protein Precursors/genetics/*metabolism Saccharomyces cerevisiae/metabolism *Saccharomyces cerevisiae Protei;" |
| Notes: | "MedlineKambe-Honjoh, H Yoda, K Yamasaki, M eng Research Support, Non-U.S. Gov't 1992/07/31 Biochem Biophys Res Commun. 1992 Jul 31; 186(2):838-45. doi: 10.1016/0006-291x(92)90822-3" |
