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Metabolites

Title:		Sampling and Analysis of Low-Molecular-Weight Volatile Metabolites in Cellular Headspace and Mouse Breath
Author(s):		Issitt T; Sweeney ST; Brackenbury WJ; Redeker KR;
Address:		"Department of Biology, University of York, York YO10 5DD, UK. York Biomedical Research Institute, University of York, York YO10 5DD, UK"
Journal Title:		Metabolites
Year:		2022
Volume:		20220627
Issue:		7
Page Number:		-
DOI:		10.3390/metabo12070599
ISSN/ISBN:		2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking)
Abstract:		"Volatile compounds, abundant in breath, can be used to accurately diagnose and monitor a range of medical conditions. This offers a noninvasive, low-cost approach with screening applications; however, the uptake of this diagnostic approach has been limited by conflicting published outcomes. Most published reports rely on large scale screening of the public, at single time points and without reference to ambient air. Here, we present a novel approach to volatile sampling from cellular headspace and mouse breath that incorporates multi-time-point analysis and ambient air subtraction revealing compound flux as an effective proxy of active metabolism. This approach to investigating breath volatiles offers a new avenue for disease biomarker discovery and diagnosis. Using gas chromatography mass spectrometry (GC/MS), we focus on low molecular weight, metabolic substrate/by-product compounds and demonstrate that this noninvasive technique is sensitive (reproducible at ~1 microg cellular protein, or ~500,000 cells) and capable of precisely determining cell type, status and treatment. Isolated cellular models represent components of larger mammalian systems, and we show that stress- and pathology-indicative compounds are detectable in mice, supporting further investigation using this methodology as a tool to identify volatile targets in human patients"
Keywords:		Voc breath breath biomarker breath diagnosis headspace volatile metabolite volatile organic compound;
Notes:		"PubMed-not-MEDLINEIssitt, Theo Sweeney, Sean T Brackenbury, William J Redeker, Kelly R eng BB/M011151/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Switzerland 2022/07/28 Metabolites. 2022 Jun 27; 12(7):599. doi: 10.3390/metabo12070599"