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Proc Natl Acad Sci U S A

Title:		A pheromone-induced developmental switch in Caenorhabditis elegans: Temperature-sensitive mutants reveal a wild-type temperature-dependent process
Author(s):		Golden JW; Riddle DL;
Address:
Journal Title:		Proc Natl Acad Sci U S A
Year:		1984
Volume:		81
Issue:		3
Page Number:		819 - 823
DOI:		10.1073/pnas.81.3.819
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"Formation of a developmentally arrested dispersal stage called the dauer larva is enhanced by a Caenorhabditis-specific pheromone and is inhibited by increasing amounts of food. Pheromone-induced dauer larva formation of three tested wild-type strains is temperature-dependent, so that an increased percentage of the population forms dauer larvae at 25 degrees C compared to lower temperatures. Dauer-defective mutants fail to respond to added pheromone, and some behavioral mutants affected in thermotaxis or egg-laying also exhibit abnormal responses. Temperature-sensitive (ts) dauer-constitutive mutants form dauer larvae at a restrictive temperature regardless of environmental stimuli. At the permissive temperature (17.5 degrees C), alleles of six out of seven dauer-constitutive genes tested overrespond to the dauer-inducing pheromone. All known mutations in daf-4 (eight alleles) and daf-7 (five alleles) produce a ts dauer-constitutive phenotype. One daf-4 and one daf-7 allele are suppressed by the amber nonsense suppressor, sup-7(st5). At least these two dauer-constitutive mutations are likely to cause production of nonfunctional rather than ts gene products. These mutations appear to indirectly result in a ts phenotype by enhancing the expression of a wild-type ts developmental process"
Keywords:		Animals Caenorhabditis/anatomy & histology/*genetics/physiology Female Larva/physiology *Mutation Oviposition Pheromones/isolation & purification/*physiology Temperature;
Notes:		"MedlineGolden, J W Riddle, D L eng HD00367/HD/NICHD NIH HHS/ HD11239/HD/NICHD NIH HHS/ N01-AG-9-2113/AG/NIA NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1984/02/01 Proc Natl Acad Sci U S A. 1984 Feb; 81(3):819-23. doi: 10.1073/pnas.81.3.819"