| Title: | "Synthesis of 'cineole cassette' monoterpenes in Nicotiana section Alatae: gene isolation, expression, functional characterization and phylogenetic analysis" |
| Author(s): | Fahnrich A; Brosemann A; Teske L; Neumann M; Piechulla B; |
| Address: | "Department of Biological Sciences, University of Rostock, Albert-Einstein-Str. 3, 18059 Rostock, Germany" |
| DOI: | 10.1007/s11103-012-9933-y |
| ISSN/ISBN: | 1573-5028 (Electronic) 0167-4412 (Linking) |
| Abstract: | "The scent bouquets of flowers of Nicotiana species, particularly those of section Alatae, are rich in monoterpenes, including 1,8-cineole, limonene, beta-myrcene, alpha- and beta-pinene, sabinene, and alpha-terpineol. New terpene synthase genes were isolated from flowers of Nicotiana bonariensis, N. forgetiana, N. longiflora, and N. mutabilis. The recombinant enzymes synthesize simultaneously the characteristic 'cineole cassette' monoterpenes with 1,8-cineole as the dominant volatile product. Interestingly, amino acid sequence comparison and phylogenetic tree construction clustered the newly isolated cineole synthases (CIN) of section Alatae together with the catalytically similar CIN of N. suaveolens of section Suaveolentes, thus suggesting a common ancestor. These CIN genes of N. bonariensis, N. forgetiana, N. longiflora, and N. mutabilis are distinct from the terpineol synthases (TERs) of the taxonomically related N. alata and N. langsdorfii (both Alatae), thus indicating gene diversification of monoterpene synthases in section Alatae. Furthermore, the presence of CINs in species of the American section Alatae supports the hypothesis that one parent of the Australian section Suaveolentes was a member of the present section Alatae. Amino acid sequences of the Nicotiana CINs and TERs were compared to identify relevant amino acids of the cyclization reaction from alpha-terpineol to 1,8-cineole" |
| Keywords: | "Amino Acid Sequence Flowers/*metabolism Gas Chromatography-Mass Spectrometry Gene Expression Regulation, Enzymologic/physiology Gene Expression Regulation, Plant/*physiology Intramolecular Lyases/chemistry/genetics/*metabolism Models, Molecular Molecular;" |
| Notes: | "MedlineFahnrich, Anke Brosemann, Anne Teske, Laura Neumann, Madeleine Piechulla, Birgit eng Research Support, Non-U.S. Gov't Netherlands 2012/06/07 Plant Mol Biol. 2012 Aug; 79(6):537-53. doi: 10.1007/s11103-012-9933-y. Epub 2012 Jun 6" |
