| Title: | A structurally deviant member of the Euplotes raikovi pheromone family: Er-23 |
| Author(s): | Di GG; Miceli C; Zahn R; Damberger F; Wuthrich K; Luporini P; |
| Address: | "Dipartimento di Biologia Molecolare Cellulare Animale, University of Camerino, Italy" |
| DOI: | 10.1111/j.1550-7408.2002.tb00347.x |
| ISSN/ISBN: | 1066-5234 (Print) 1066-5234 (Linking) |
| Abstract: | "Pheromones of Euplotes raikovi form a homologous family of proteins with 37- to 40-amino acid residues, including six cysteines that form three strictly conserved disulfide bridges. The determination of the primary structure of the pheromone Er-23, which was isolated from cells derived from natural populations of E. raikovi that secrete the other known pheromones, has now revealed a novel structure type. The polypeptide chain of this pheromone contains 51 residues, 10 of which are cysteines presumably involved in the formation of five disulfide bridges, and lacks a carboxyl-terminal tail following the last cysteine of the sequence. The elongation of the Er-23 molecule is presumed to result from multiple events of gene duplication starting from an ancestral motif Xxx(2-4)-Cys-Xxx(5-7)-Cys" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Electrophoresis, Capillary Euplotes/*genetics Molecular Sequence Data Nucleic Acid Conformation Pheromones/*chemistry/genetics/isolation & purification Protozoan Proteins/*chemistry/genetics/isolation & purificati;" |
| Notes: | "MedlineDi, Giuseppe Graziano Miceli, Cristina Zahn, Ralph Damberger, Fred Wuthrich, Kurt Luporini, Pierangelo eng Comparative Study Research Support, Non-U.S. Gov't 2002/03/23 J Eukaryot Microbiol. 2002 Jan-Feb; 49(1):86-92. doi: 10.1111/j.1550-7408.2002.tb00347.x" |
