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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		"Quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, Canis lupus familiaris"
Author(s):		de Oliveira Filho JG; Sarria ALF; Ferreira LL; Caulfield JC; Powers SJ; Pickett JA; de Leon AAP; Birkett MA; Borges LMF;
Address:		"Escola de Veterinaria e Zootecnia, Universidade Federal de Goias, Campus II, Samambaia, Cx. Postal 131, Goiania, Goias CEP: 74001-970, Brazil. Electronic address: jaires_filho@hotmail.com. Biological Chemistry and Crop Protection Department, Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, United Kingdom. Electronic address: andre.sarria@rothamsted.ac.uk. Escola de Veterinaria e Zootecnia, Universidade Federal de Goias, Campus II, Samambaia, Cx. Postal 131, Goiania, Goias CEP: 74001-970, Brazil. Electronic address: loren4_lopes@hotmail.com. Biological Chemistry and Crop Protection Department, Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, United Kingdom. Electronic address: john.caulfield@rothamsted.ac.uk. Computational and Systems Biology Department, Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, United Kingdom. Electronic address: stephen.powers@rothamsted.ac.uk. Biological Chemistry and Crop Protection Department, Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, United Kingdom. Electronic address: john.pickett@rothamsted.ac.uk. USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory, Veterinary Pest Genomics Center, 2700 Fredericksburg Road, Kerrville, TX 78028, USA. Electronic address: beto.perezdeleon@ars.usda.gov. Biological Chemistry and Crop Protection Department, Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, United Kingdom. Electronic address: mike.birkett@rothamsted.ac.uk. Instituto de Patologia Tropical e Saude Publica, Universidade Federal de Goias, Rua 235 s/n, Setor Universitario, Goiania, Goias CEP: 74605050, Brazil. Electronic address: borges.ligia@gmail.com"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2016
Volume:		20160316
Issue:
Page Number:		64 - 69
DOI:		10.1016/j.jchromb.2016.03.014
ISSN/ISBN:		1873-376X (Electronic) 1570-0232 (Linking)
Abstract:		"We have recently shown that repellency of the tick Rhipicephalus sanguineus sensu lato by the tick resistant dog breed, the beagle, is mediated by volatile organic compounds (VOCs) 2-hexanone and benzaldehyde present in beagle odour. Ectoparasite location of animal hosts is affected by variation in these odour components and their ratios. The aim of this study was to quantify the release rate, and the ratio, of 2-hexanone and benzaldehyde from beagles. The odour of three beagles was collected, for four days, over one week (day 0, day 1, day 4 and day 7). The compounds were identified using coupled high-resolution gas chromatography-mass spectrometry (GC-MS), and authentic standards of compounds were used to generate external calibration curves for quantification. Both compounds were found in all dogs on all days. The amount of benzaldehyde was always higher than that of 2-hexanone and so their ratio varied from unity, on average (over time) being 3.128+/-0.365, 1.902+/-0.390, 1.670+/-0.671ngmL(-1) for beagle 1, 2 and 3, respectively. There was no significant (p<0.05, F-test) effect of time. The overall mean was 2.233+/-0.387ngmL(-1). These results further previous findings by documenting the presence of 2-hexanone and benzaldehyde in beagle odour samples covering a 7-day period. This knowledge enables development of repellents to protect dogs from R. sanguineus s. l. infestation"
Keywords:		Animals Benzaldehydes/*analysis/*pharmacology Dogs/*parasitology Gas Chromatography-Mass Spectrometry Limit of Detection Linear Models Methyl n-Butyl Ketone/*analysis/*pharmacology Reproducibility of Results Rhipicephalus sanguineus/*drug effects 2-Hexano;
Notes:		"Medlinede Oliveira Filho, Jaires Gomes Sarria, Andre Lucio Franceschini Ferreira, Lorena Lopes Caulfield, John C Powers, Stephen J Pickett, John A de Leon, Adalberto A Perez Birkett, Michael A Borges, Ligia Miranda Ferreira eng BBS/E/C/00004944/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Netherlands 2016/04/17 J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Jun 1; 1022:64-69. doi: 10.1016/j.jchromb.2016.03.014. Epub 2016 Mar 16"