| Title: | Potential of on-line CIMS for bioprocess monitoring |
| Author(s): | Custer TG; Wagner WP; Kato S; Bierbaum VM; Fall R; |
| Address: | "Department of Chemistry and Biochemistry, University of Colorado, Boulder Colorado 80309-0215, USA" |
| ISSN/ISBN: | 8756-7938 (Print) 1520-6033 (Linking) |
| Abstract: | "Chemical-ionization mass spectrometry (CIMS) using flow reactors is an emerging method for on-line monitoring of trace concentrations of organic compounds in the gas phase. In this study, a flow-reactor CIMS instrument, employing the H(3)O(+) cation as the ionizing reagent, was used to simultaneously monitor several volatile metabolic products as they are released into the headspace during bacterial growth in a bioreactor. Production of acetaldehyde, ethanol, acetone, butanol, acetoin, diacetyl, and isoprene by Bacillus subtilis is reported. Ion signal intensities were related to solution-phase concentrations using empirical calibrations and, in the case of isoprene, were compared with simultaneous gas chromatography measurements. Identification of volatile and semivolatile metabolites is discussed. Flow-reactor CIMS techniques should be useful for bioprocess monitoring applications because of their ability to sensitively and simultaneously monitor many volatile metabolites on-line" |
| Keywords: | Acetoin/*metabolism Bacillus subtilis/*growth & development/*metabolism Bioreactors/*microbiology Butadienes/*metabolism Cell Culture Techniques/*instrumentation/methods Equipment Design Equipment Failure Analysis Feasibility Studies Flow Injection Analys; |
| Notes: | "MedlineCuster, Thomas G Wagner, William P Kato, Shuji Bierbaum, Veronica M Fall, Ray eng Evaluation Study Research Support, U.S. Gov't, Non-P.H.S. Validation Study 2003/08/02 Biotechnol Prog. 2003 Jul-Aug; 19(4):1355-64. doi: 10.1021/bp025730k" |
