| Title: | Screening antagonistic yeasts against citrus green mold and the possible biocontrol mechanisms of Pichia galeiformis (BAF03) |
| Author(s): | Chen O; Yi L; Deng L; Ruan C; Zeng K; |
| Address: | "College of Food Science, Southwest University, Chongqing, China. Food Storage and Logistics Research Center, Southwest University, Chongqing, China" |
| ISSN/ISBN: | 1097-0010 (Electronic) 0022-5142 (Linking) |
| Abstract: | "BACKGROUND: Penicillium digitatum is one of the most important pathogens causing citrus green mold, leading to significant economic losses. Traditionally, synthetic fungicides are used to control diseases. However, the side effects of fungicides should not be ignored. Thus, antagonistic yeasts were proposed to be safe and effective alternatives for managing diseases. Orchards are excellent sources of naturally occurring antagonists against pathogens. Therefore, in the present study, antagonistic yeasts obtained from orchards were screened, and the possible biocontrol mechanisms of the most promising yeast were investigated. RESULTS: Seventy-eight isolates of yeasts (15 species of 10 genera) were obtained from citrus orchards. In in vitro assays, 16 strains showed antifungal activity against Pichia digitatum and 15 strains showed biocontrol potential against green mold on Olinda oranges. Pichia galeiformis (BAF03) exhibited the best antagonistic activity against P. digitatum during 6 days storage at 25 degrees C and a good antagonistic activity during 29 days at 4 degrees C. Pichia galeiformis (BAF03) could colonize and amplify quickly in wounded citrus. Scanning electron microscopy results showed that the citrus wound was colonised by the yeast. A total of eight volatile organic compounds (VOCs) were identified by gas chromatography-mass spectrometry The VOCs produced by P. galeiformis (BAF03) efficiently inhibited P. digitatum. CONCLUSION: Pichia galeiformis (BAF03) isolated from a citrus orchard showed potential to control postharvest green mold of citrus. The possible mechanisms of action likely include competition for space and nutrients as well as production of VOCs" |
| Keywords: | *Antibiosis Citrus/*microbiology Penicillium/*physiology Pichia/*physiology Plant Diseases/*microbiology/prevention & control Penicillium digitatum biocontrol citrus mechanism yeast; |
| Notes: | "MedlineChen, Ou Yi, Lanhua Deng, Lili Ruan, Changqing Zeng, Kaifang eng 2018YFD0401301/National Key R&D Program of China/ 31772027/National Natural Science Foundation of China/ England 2020/04/06 J Sci Food Agric. 2020 Aug; 100(10):3812-3821. doi: 10.1002/jsfa.10407. Epub 2020 May 28" |
