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Mol Cell Biol

Title:		Role of the transcription activator Ste12p as a repressor of PRY3 expression
Author(s):		Bickel KS; Morris DR;
Address:		"Department of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195, USA"
Journal Title:		Mol Cell Biol
Year:		2006
Volume:		20060828
Issue:		21
Page Number:		7901 - 7912
DOI:		10.1128/MCB.01004-06
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"Mating pheromone represses synthesis of full-length PRY3 mRNA, and a new transcript appears simultaneously with its 5' terminus 452 nucleotides inside the open reading frame (ORF). Synthesis of this shorter transcript results from activation of a promoter within the PRY3 locus, and its production is concomitant with the rapid disappearance of the full-length transcript. Evidence is consistent with the pheromone-induced transcription factor Ste12p binding two pheromone response elements within the PRY3 promoter, directly impeding transcription of the full-length mRNA while simultaneously inducing initiation of the short transcript. This process depends on a TATA box within the PRY3 ORF. Expression of full-length PRY3 inhibited mating, while no disadvantage was detectable for cells unable to make the short transcript. Therefore, Ste12p is utilized as a repressor of full-length PRY3 transcription, ensuring efficient mating. There is no evidence that production of the short PRY3 transcript is anything more than an adventitious by-product of this mechanism. It is possible that cryptic binding sites for transcriptional activators may occur frequently within genomes and have the potential of evolving for rapid, gene-specific repression by mechanisms analogous to PRY3. PRY3 regulation provides a model for the coordination of both inductive and repressive activities within a regulatory network"
Keywords:		"5' Flanking Region DNA-Binding Proteins/metabolism *Gene Expression Regulation, Fungal Open Reading Frames Pheromones/*genetics/metabolism Promoter Regions, Genetic RNA Polymerase II/antagonists & inhibitors/metabolism RNA, Messenger/metabolism Regulatory;"
Notes:		"MedlineBickel, Kellie S Morris, David R eng T32 GM07270/GM/NIGMS NIH HHS/ T32 GM007270/GM/NIGMS NIH HHS/ CA71453/CA/NCI NIH HHS/ R01 CA071453/CA/NCI NIH HHS/ CA89807/CA/NCI NIH HHS/ R33 CA089807/CA/NCI NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. 2006/08/31 Mol Cell Biol. 2006 Nov; 26(21):7901-12. doi: 10.1128/MCB.01004-06. Epub 2006 Aug 28"