| Title: | Protocol for isolation and analysis of small volatile microbiome metabolites from human or mouse samples |
| Author(s): | Bell HN; Das NK; Shah YM; |
| Address: | "Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA. University of Michigan Rogel Cancer Center, University of Michigan, Ann Arbor, MI, USA. Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA" |
| DOI: | 10.1016/j.xpro.2022.101311 |
| ISSN/ISBN: | 2666-1667 (Electronic) 2666-1667 (Linking) |
| Abstract: | "Metabolites are crucial for bidirectional communication between host and microbiome. We describe a protocol for the isolation of organic and aqueous metabolites from mucosal scrapes and feces from mouse and human samples. Although some of the most reactive organic compounds may be lost, this approach generates a functionally reproducible metabolic extract containing both host and microbial compounds appropriate for quantitative mass spectrometry and functional characterization. Our mass spectrometry approach identifies low-abundant and difficult to identify microbially derived metabolites. For complete details on the use and execution of this protocol, please refer to Bell et al. (2021) and Das et al. (2020)" |
| Keywords: | Animals Feces/chemistry Humans Mass Spectrometry/methods Mice *Microbiota Organic Chemicals/analysis Mass Spectrometry Metabolism Metabolomics Microbiology Molecular Biology; |
| Notes: | "MedlineBell, Hannah N Das, Nupur K Shah, Yatrik M eng R01 CA148828/CA/NCI NIH HHS/ R01 DK095201/DK/NIDDK NIH HHS/ R01 CA245546/CA/NCI NIH HHS/ F30 CA257292/CA/NCI NIH HHS/ T32 GM007863/GM/NIGMS NIH HHS/ P50 CA130810/CA/NCI NIH HHS/ P30 CA046592/CA/NCI NIH HHS/ T32 GM007315/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. 2022/05/03 STAR Protoc. 2022 Apr 13; 3(2):101311. doi: 10.1016/j.xpro.2022.101311. eCollection 2022 Jun 17" |
