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Biochem Biophys Res Commun

Title:		Production of a soluble and secreted antigenic fragment of HBsAg in yeast
Author(s):		Baba EH; Berkower I;
Address:		"Departamento de Bioquimica-ICB-UFMG, ICEB-Universidade Federal de Ouro Preto, Brazil"
Journal Title:		Biochem Biophys Res Commun
Year:		1992
Volume:		184
Issue:		1
Page Number:		50 - 59
DOI:		10.1016/0006-291x(92)91156-k
ISSN/ISBN:		0006-291X (Print) 0006-291X (Linking)
Abstract:		"We have produced a fragment of hepatitis B surface antigen (HBsAg) corresponding to amino acids 1-60 as a fusion protein with the alpha mating factor of yeast. The product is secreted from yeast as a soluble monomer that expresses HBsAg antigenicity. Unlike other heterologous fusion proteins, it is not processed by the Lys-Arg endoprotease, possibly due to a proline in the linker between the two coding sequences. The resulting soluble fragment will enable us to map the immunodominant sites of HBsAg recognized by T cells and to identify additional factors contributing to vaccine potency"
Keywords:		"Amino Acid Sequence Base Sequence DNA, Viral/genetics/isolation & purification Escherichia coli/genetics Genetic Vectors Hepatitis B Surface Antigens/biosynthesis/*genetics/isolation & purification Hepatitis B virus/genetics Mating Factor Molecular Sequen;"
Notes:		"MedlineBaba, E H Berkower, I eng 1992/04/15 Biochem Biophys Res Commun. 1992 Apr 15; 184(1):50-9. doi: 10.1016/0006-291x(92)91156-k"