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J Biol Chem

Title:		Purification and characterization of a paired basic residue-specific yeast aspartic protease encoded by the YAP3 gene. Similarity to the mammalian pro-opiomelanocortin-converting enzyme
Author(s):		Azaryan AV; Wong M; Friedman TC; Cawley NX; Estivariz FE; Chen HC; Loh YP;
Address:		"Section on Cellular Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892"
Journal Title:		J Biol Chem
Year:		1993
Volume:		268
Issue:		16
Page Number:		11968 - 11975
DOI:
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"Yeast cells express an alternate enzyme encoded by the YAP3 gene which can process pro-alpha-mating factor when this pheromone is overexpressed in KEX2-deficient mutants. The YAP3 gene product is an aspartic protease (YAP3) that cleaves at paired basic residues (Egel-Mittani, M., Flygenring, H.P., and Hansen, M. T. (1990) Yeast 6, 127-137). In this study, the YAP3 gene was overexpressed in the BJ 3501 strain of Saccharomyces cerevisiae. YAP3 was purified to apparent homogeneity using concanavalin A and pepstatin A affinity chromatography. The enzyme was characterized as an M(r) 68,000 glycoprotein with a pH optimum of 4.0-4.5. It was inhibited by pepstatin A and activated by 5 mM Ca2+. YAP3 cleaved at paired basic residues of mouse pro-opiomelanocortin (POMC) to yield adrenocorticotropin (ACTH) and beta-lipotropin (LPH); human beta-LPH to yield beta-endorphin-(1-31), beta-endorphin-(1-29), beta-endorphin-(1-28), gamma-LPH, and beta-melanocyte-stimulating hormone; and bovine N-POMC1-77 to yield gamma 3-melanocyte-stimulating hormone. It also cleaved the tetrabasic residues of ACTH1-39 to yield primarily ACTH1-15 and Lys-Arg-corticotropin-like intermediate lobe peptide. The physical properties, pH optimum, and specificity of YAP3 indicate that it is a homologue of the mammalian POMC-converting enzyme (EC 3.4.23.17), a paired basic residue-specific aspartic protease from bovine pituitary intermediate lobe secretory granules (Loh, Y. P., Parish, D.C., and Tuteja, R. (1985) J. Biol. Chem. 260, 7194-7205)"
Keywords:		"Adrenocorticotropic Hormone/metabolism Amino Acid Sequence Animals Aspartic Acid Endopeptidases/*genetics/isolation & purification/*metabolism Cattle Chromatography, Affinity Endopeptidases/*metabolism *Genes, Fungal Humans Kinetics Mice Molecular Sequenc;"
Notes:		"MedlineAzaryan, A V Wong, M Friedman, T C Cawley, N X Estivariz, F E Chen, H C Loh, Y P eng Research Support, U.S. Gov't, P.H.S. 1993/06/05 J Biol Chem. 1993 Jun 5; 268(16):11968-75"