| Title: | Proteomic Characterization of Drosophila melanogaster Proboscis |
| Author(s): | Aruci E; Saliou JM; Ferveur JF; Briand L; |
| Address: | "Centre des Sciences du Gout et de l'Alimentation, CNRS, INRAE, Institut Agro, Universite Bourgogne Franche-Comte, 21000 Dijon, France. CNRS, INSERM CHU Lille, Institut Pasteur de Lille, UAR CNRS 2014-US Inserm 41-PLBS, University of Lille, 59000 Lille, France" |
| ISSN/ISBN: | 2079-7737 (Print) 2079-7737 (Electronic) 2079-7737 (Linking) |
| Abstract: | "Drosophila melanogaster flies use their proboscis to taste and distinguish edible compounds from toxic compounds. With their proboscis, flies can detect sex pheromones at a close distance or by contact. Most of the known proteins associated with probosci's detection belong to gustatory receptor families. To extend our knowledge of the proboscis-taste proteins involved in chemo-detection, we used a proteomic approach to identify soluble proteins from Drosophila females and males. This investigation, performed with hundreds of dissected proboscises, was initiated by the chromatographic separation of tryptic peptides, followed by tandem mass spectrometry, allowing for femtomole detection sensitivity. We found 586 proteins, including enzymes, that are involved in intermediary metabolism and proteins dedicated to various functions, such as nucleic acid metabolism, ion transport, immunity, digestion, and organ development. Among 60 proteins potentially involved in chemosensory detection, we identified two odorant-binding proteins (OBPs), i.e., OBP56d (which showed much higher expression in females than in males) and OBP19d. Because OBP56d was also reported to be more highly expressed in the antennae of females, this protein can be involved in the detection of both volatile and contact male pheromone(s). Our proteomic study paves the way to better understand the complex role of Drosophila proboscis in the chemical detection of food and pheromonal compounds" |
| Keywords: | chemosensory system mass spectrometry odorant-binding proteins proteomics taste; |
| Notes: | "PubMed-not-MEDLINEAruci, Enisa Saliou, Jean-Michel Ferveur, Jean-Francois Briand, Loic eng 0/National Research Institute for Agriculture, Food and Environment/ Switzerland 2022/11/25 Biology (Basel). 2022 Nov 21; 11(11):1687. doi: 10.3390/biology11111687" |
