| Title: | Volatile profiles of human skin cell cultures in different degrees of senescence |
| Author(s): | Acevedo CA; Sanchez EY; Reyes JG; Young ME; |
| Address: | "Universidad Tecnica Federico Santa Maria, Centro de Biotecnologia, Avenida Espana 1680, Valparaiso, Chile. cristian.acevedo@usm.cl" |
| Journal Title: | J Chromatogr B Analyt Technol Biomed Life Sci |
| DOI: | 10.1016/j.jchromb.2009.12.033 |
| ISSN/ISBN: | 1873-376X (Electronic) 1570-0232 (Linking) |
| Abstract: | "It is known that skin releases volatile organic compounds to the environment, and also that its emission pattern changes with aging of the skin. It could be considered, that these compounds are intermediaries in cell metabolism, since many intermediaries of metabolic pathways have a volatile potential. In this work, a simple and non-destructive method consisting of SPME sampling and GC/MS analysis was developed to identify volatile organic emanations from cell cultures. This technique, applied to skin cells culture, indicates that the cells or cell metabolism produce several skin emissions. Chemometric analysis was performed in order to explore the relationship between a volatile profile and the senescence of cell cultures. Volatile profiles were different for cell cultures in different degrees of senescence, indicating that volatile compound patterns could be used to provide information about the age of skin cells" |
| Keywords: | "Cells, Cultured *Cellular Senescence Child Child, Preschool Computational Biology Gas Chromatography-Mass Spectrometry Humans Least-Squares Analysis Metabolic Networks and Pathways Principal Component Analysis Skin/*cytology/*metabolism Solid Phase Microe;" |
| Notes: | "MedlineAcevedo, Cristian A Sanchez, Elizabeth Y Reyes, Juan G Young, Manuel E eng Research Support, Non-U.S. Gov't Netherlands 2010/01/12 J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Feb 1; 878(3-4):449-55. doi: 10.1016/j.jchromb.2009.12.033. Epub 2010 Jan 4" |
