| Title: | Restricted Access Volatile Supramolecular Solvents for Single-Step Extraction/Cleanup of Benzimidazole Anthelmintic Drugs in Milk Prior to LC-MS/MS |
| Author(s): | Accioni F; Caballero-Casero N; Garcia-Gomez D; Rubio S; |
| Address: | "Department of Analytical Chemistry, Institute of Fine Chemistry and Nanochemistry , Universidad de Cordoba , Cordoba 14071 , Spain. Department of Chemistry and Pharmacy , University of Sassari , 07100 Sassari SS , Italy" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "In this work, a restricted access volatile supramolecular solvent (RAM-VOL-SUPRAS) directly synthesized in milk is proposed for the first time for the simultaneous extraction and cleanup of benzimidazole anthelmintic drugs in milk meant for human consumption. The RAM-VOL-SUPRAS was formed by the self-assembly and coacervation of hexanol in tetrahydrofuran induced by the water content in milk. Benzimidazoles legislated by the European Union were quantitatively extracted (80-110%), and proteins were precipitated by the action of THF and the amphiphile; extraction of carbohydrates was avoided by a size exclusion mechanism, and lipids were removed during hexanol evaporation. The analytical methodology was fully validated according to Commission Decision 2002/657/EC. Method detection limits from 0.03 to 0.14 mug L(-1) were well below the maximum residue limits legislated in milk for these drugs, with interday precisions at maximum residue levels below 13%. This novel methodology guarantees a rapid and reliable tool for daily and routine laboratory analyses in the field of food quality control" |
| Keywords: | "Animals Anthelmintics/*analysis/*isolation & purification Benzimidazoles/*analysis/*isolation & purification Cattle Chromatography, Liquid Food Contamination/analysis Furans/chemistry Hexanols/chemistry Limit of Detection Milk/*chemistry Solid Phase Extra;" |
| Notes: | "MedlineAccioni, Francesca Caballero-Casero, Noelia Garcia-Gomez, Diego Rubio, Soledad eng Evaluation Study 2018/12/06 J Agric Food Chem. 2019 Jan 9; 67(1):520-530. doi: 10.1021/acs.jafc.8b06003. Epub 2018 Dec 19" |
