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Front Microbiol

Title:		Identification and Functional Analysis of the Pheromone Response Factor Gene of Sporisorium scitamineum
Author(s):		Zhu G; Deng Y; Cai E; Yan M; Cui G; Wang Z; Zou C; Zhang B; Xi P; Chang C; Chen B; Jiang Z;
Address:		"Guangdong Province Key Laboratory of Microbial Signals and Disease Control, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Department of Plant Pathology, South China Agricultural University, Guangzhou, China. Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, China. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning, China"
Journal Title:		Front Microbiol
Year:		2019
Volume:		20190910
Issue:
Page Number:		2115 -
DOI:		10.3389/fmicb.2019.02115
ISSN/ISBN:		1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking)
Abstract:		"The sugarcane smut fungus Sporisorium scitamineum is bipolar and produces sporidia of two different mating types. During infection, haploid cells of opposite mating types can fuse to form dikaryotic hyphae that can colonize plant tissue. Mating and filamentation are therefore essential for S. scitamineum pathogenesis. In this study, we obtained one T-DNA insertion mutant disrupted in the gene encoding the pheromone response factor (Prf1), hereinafter named SsPRF1, of S. scitamineum, via Agrobacterium tumefaciens-mediated transformation (ATMT) mutagenesis. Targeted deletion of SsPRF1 resulted in mutants with phenotypes similar to the T-DNA insertion mutant, including failure to mate with a compatible wild-type partner strain and being non-pathogenic on its host sugarcane. qRT-PCR analyses showed that SsPRF1 was essential for the transcription of pheromone-responsive mating type genes of the a1 locus. These results show that SsPRF1 is involved in mating and pathogenicity and plays a key role in pheromone signaling and filamentous growth in S. scitamineum"
Keywords:		Sporisorium scitamineum fungi mating pathogenicity pheromone response factor;
Notes:		"PubMed-not-MEDLINEZhu, Guining Deng, Yizhen Cai, Enping Yan, Meixin Cui, Guobing Wang, Zhiqiang Zou, Chengwu Zhang, Bin Xi, Pinggen Chang, Changqing Chen, Baoshan Jiang, Zide eng Switzerland 2019/09/26 Front Microbiol. 2019 Sep 10; 10:2115. doi: 10.3389/fmicb.2019.02115. eCollection 2019"