« Previous AbstractOdorant receptors from Culex quinquefasciatus and Aedes aegypti sensitive to floral compounds    Next AbstractDetection and Discrimination of Volatile Organic Compounds using a Single Film Bulk Acoustic Wave Resonator with Temperature Modulation as a Multiparameter Virtual Sensor Array »

Biochem Biophys Res Commun

Title:		Analysis of a cDNA library from the antenna of Cnaphalocrocis medinalis and the expression pattern of olfactory genes
Author(s):		Zeng FF; Sun X; Dong HB; Wang MQ;
Address:		"Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China"
Journal Title:		Biochem Biophys Res Commun
Year:		2013
Volume:		20130322
Issue:		4
Page Number:		463 - 469
DOI:		10.1016/j.bbrc.2013.03.038
ISSN/ISBN:		1090-2104 (Electronic) 0006-291X (Linking)
Abstract:		"Chemoreception is a key feature in selection of host plants by insects. In this study, preliminary characterization and isolation of cDNA clones from Cnaphalocrocis medinalis antennal libraries identified eight olfactory genes, including two putative general odorant-binding proteins (GOBPs), three pheromone binding proteins (PBPs) and three chemosensory proteins (CSPs). The expression profiles of these eight genes in different tissues (antenna, head (without antennae), thorax, abdomen, leg and wing) were measured by real time qPCR. The results showed that GOBP and PBP genes in C. medinalis seemed to be antenna-specific, but differentially expressed in male and female antennae; while CSP genes were expressed ubiquitously during different developmental stages, but with an extremely elevated transcript level in antennae, legs and wings compared to head, thorax and abdomen. And also, the transcription levels of olfactory genes depended on the age, sex, and mating status of the adults. These findings support the hypothesis that OBPs and CSPs play dynamic roles during development of C. medinalis and are likely to be involved in broader physiological functions"
Keywords:		"Amino Acid Sequence Animals Arthropod Antennae/*cytology/metabolism Cloning, Molecular Computational Biology Female *Gene Expression Regulation, Developmental Gene Library *Genes, Insect Insect Proteins/genetics/metabolism Larva/cytology/genetics/growth &;"
Notes:		"MedlineZeng, Fang-Fang Sun, Xiao Dong, Hai-Bo Wang, Man-Qun eng Research Support, Non-U.S. Gov't 2013/03/26 Biochem Biophys Res Commun. 2013 Apr 19; 433(4):463-9. doi: 10.1016/j.bbrc.2013.03.038. Epub 2013 Mar 22"