| Title: | Expansion of sweet taste receptor genes in grass carp (Ctenopharyngodon idellus) coincided with vegetarian adaptation |
| Author(s): | Yuan XC; Liang XF; Cai WJ; He S; Guo WJ; Mai KS; |
| Address: | "College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, China. Innovation Base for Chinese Perch Breeding, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China. College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, China. xufang_liang@hotmail.com. Innovation Base for Chinese Perch Breeding, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, 430070, China. xufang_liang@hotmail.com. The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, Shandong, China" |
| DOI: | 10.1186/s12862-020-1590-1 |
| ISSN/ISBN: | 1471-2148 (Electronic) 1471-2148 (Linking) |
| Abstract: | "BACKGROUND: Taste is fundamental to diet selection in vertebrates. Genetic basis of sweet taste receptor in the shaping of food habits has been extensively studied in mammals and birds, but scarcely studied in fishes. Grass carp is an excellent model for studying vegetarian adaptation, as it exhibits food habit transition from carnivory to herbivory. RESULTS: We identified six sweet taste receptors (gcT1R2A-F) in grass carp. The four gcT1R2s (gcT1R2C-F) have been suggested to be evolved from and paralogous to the two original gcT1R2s (gcT1R2A and gcT1R2B). All gcT1R2s were expressed in taste organs and mediated glucose-, fructose- or arginine-induced intracellular calcium signaling, revealing they were functional. In addition, grass carp was performed to prefer fructose to glucose under a behavioral experiment. Parallelly, compared with gcT1R2A-F/gcT1R3 co-transfected cells, gcT1R2C-F/gcT1R3 co-transfected cells showed a higher response to plant-specific fructose. Moreover, food habit transition from carnivory to herbivory in grass carp was accompanied by increased gene expression of certain gcT1R2s. CONCLUSIONS: We suggested that the gene expansion of T1R2s in grass carp was an adaptive strategy to accommodate the change in food environment. Moreover, the selected gene expression of gcT1R2s might drive the food habit transition from carnivory to herbivory in grass carp. This study provided some evolutional and physiological clues for the formation of herbivory in grass carp" |
| Keywords: | "Acclimatization/genetics Adaptation, Biological/*genetics Animals Carps/classification/*genetics/physiology Feeding Behavior Fish Proteins/genetics Gene Amplification/physiology Gene Expression Herbivory/*genetics Mammals/genetics Receptors, G-Protein-Cou;" |
| Notes: | "MedlineYuan, Xiao-Chen Liang, Xu-Fang Cai, Wen-Jing He, Shan Guo, Wen-Jie Mai, Kang-Sen eng Research Support, Non-U.S. Gov't England 2020/02/13 BMC Evol Biol. 2020 Feb 11; 20(1):25. doi: 10.1186/s12862-020-1590-1" |
