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Biochem Biophys Res Commun

Title:		Identification and expression pattern analysis of BoMYB51 involved in indolic glucosinolate biosynthesis from broccoli (Brassica oleracea var. italica)
Author(s):		Yu Q; Hao G; Zhou J; Wang J; Evivie ER; Li J;
Address:		"College of Life Sciences, Northeast Agricultural University, China. College of Life Sciences, Northeast Agricultural University, China. Electronic address: lijing@neau.edu.cn"
Journal Title:		Biochem Biophys Res Commun
Year:		2018
Volume:		501
Issue:		2
Page Number:		598 - 604
DOI:		10.1016/j.bbrc.2018.05.058
ISSN/ISBN:		1090-2104 (Electronic) 0006-291X (Linking)
Abstract:		"Glucosinolates are a class of amino acid-derived specialized metabolites characteristic of the Brassicales order. Trp derived indolic glucosinolates are essential for the effective plant defense responses to a wide range of pathogens and herbivores. In Arabidopsis, MYB51 is the key transcription factor positively regulates indolic glucosinolate production by activating certain biosynthetic genes. In this study, we report the isolation and identification of a MYB51 from broccoli designated as BoMYB51. Overexpression of BoMYB51 in Arabidopsis increased indolic glucosinolate production by upregulating biosynthetic genes and resulted in enhanced flagellin22 (Flg22) induced callose deposition. The spatial expression pattern and responsive expression of BoMYB51 to several hormones and stress treatments were investigated by expressing the beta-glucuronidase (GUS) reporter gene driven by BoMYB51 promotor in Arabidopsis and quantitative real-time PCR analysis in broccoli. Our study provides information on molecular characteristics of BoMYB51 and possible physiological process BoMYB51 may involve"
Keywords:		"Arabidopsis/genetics/metabolism Biosynthetic Pathways Brassica/*genetics/metabolism Cloning, Molecular *Gene Expression Regulation, Plant Genes, Plant Glucosinolates/*genetics/metabolism Plant Proteins/*genetics/metabolism Transcription Factors/*genetics/;"
Notes:		"MedlineYu, Qingyue Hao, Guodong Zhou, Jianxin Wang, Jingying Evivie, Ejiroghene Ruona Li, Jing eng Research Support, Non-U.S. Gov't 2018/05/14 Biochem Biophys Res Commun. 2018 Jun 22; 501(2):598-604. doi: 10.1016/j.bbrc.2018.05.058"