| Title: | The MAT1 locus of Histoplasma capsulatum is responsive in a mating type-specific manner |
| Address: | "Department of Pathology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0560, USA" |
| ISSN/ISBN: | 1535-9778 (Print) 1535-9786 (Electronic) 1535-9786 (Linking) |
| Abstract: | "Recombination events associated with sexual replication in pathogens may generate new strains with altered virulence. Histoplasma capsulatum is a mating-competent, pathogenic fungus with two described phenotypic mating types, + and -. The mating (MAT) locus of H. capsulatum was identified to facilitate molecular studies of mating in this organism. Through syntenic analysis of the H. capsulatum genomic sequence databases, a MAT1-1 idiomorph region was identified in H. capsulatum strains G217B and WU24, and a MAT1-2 idiomorph region was identified in the strain G186AR. A mating type-specific PCR assay was developed, and two clinical isolates of opposite genotypic mating type, UH1 and VA1, were identified. A known--mating type strain, T-3-1 (ATCC 22635), was demonstrated to be of MAT1-2 genotypic mating type. The clinical isolates UH1 and VA1 were found to be mating compatible and also displayed mating-type-dependent regulation of the MAT transcription factors in response to extracts predicted to contain mating pheromones. These studies support a role for the identified MAT1 locus in determining mating type in H. capsulatum" |
| Keywords: | "Amino Acid Sequence Fungal Proteins/chemistry/genetics/metabolism Gene Expression Regulation, Fungal/*physiology Genes, Mating Type, Fungal/*genetics Histoplasma/*cytology/*genetics Molecular Sequence Data Polymerase Chain Reaction Transcription Factors/g;" |
| Notes: | "MedlineBubnick, Meggan Smulian, A George eng 2007/02/27 Eukaryot Cell. 2007 Apr; 6(4):616-21. doi: 10.1128/EC.00020-07. Epub 2007 Feb 23" |
