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Bull Entomol Res

Title:		"AlepPBP2, but not AlepPBP3, may involve in the recognition of sex pheromones and maize volatiles in Athetis lepigone"
Author(s):		Yang HH; Xu JW; Zhang XQ; Huang JR; Li LL; Yao WC; Zhao PP; Zhang D; Liu JY; Dewer Y; Zhu XY; Li XM; Zhang YN;
Address:		"Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Anhui Provincial Engineering Laboratory for Efficient Utilization of Featured Resource Plants, College of Life Sciences, Huaibei Normal University, Huaibei, China. Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, College of Plant Protection, Nanjing Agricultural University, Nanjing, China. Henan Key Laboratory of Crop Pest Control, MOA's Regional Key Lab of Crop IPM in Southern Part of Northern China, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, China. College of Information, Huaibei Normal University, Huaibei, China. Phytotoxicity Research Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, 7 Nadi El-Seid Street, Dokki 12618, Giza, Egypt"
Journal Title:		Bull Entomol Res
Year:		2022
Volume:		20220224
Issue:		4
Page Number:		536 - 545
DOI:		10.1017/S0007485321001127
ISSN/ISBN:		1475-2670 (Electronic) 0007-4853 (Linking)
Abstract:		"Athetis lepigone Moschler (Lepidoptera, Noctuidae) is a common maize pest in Europe and Asia. However, there is no long-term effective management strategy is available yet to suppress its population. Adults rely heavily on olfactory cues to locate their optimal host plants and oviposition sites. Pheromone-binding proteins (PBPs) are believed to be responsible for recognizing and transporting different odorant molecules to interact with receptor membrane proteins. In this study, the ligand-binding specificities of two AlepPBPs (AlepPBP2 and AlepPBP3) for sex pheromone components and host plant (maize) volatiles were measured by fluorescence ligand-binding assay. The results demonstrated that AlepPBP2 had a high affinity with two pheromones [(Z)-7-dodecenyl acetate, Ki = 1.11 +/- 0.1 muM, (Z)-9-tetradecenyl acetate, Ki = 1.32 +/- 0.15 muM] and ten plant volatiles, including (-)-limonene, alpha-pinene, myrcene, linalool, benzaldehyde, nonanal, 2-hexanone, 3-hexanone, 2-heptanone and 6-methyl-5-hepten-2-one. In contrast, we found that none of these chemicals could bind to AlepPBP3. Our results clearly show no significant differences in the functional characterization of the binding properties between AlepPBP2 and AlepPBP3 to sex pheromones and host plant volatiles. Furthermore, molecular docking was employed for further detail on some crucial amino acid residues involved in the ligand-binding of AlepPBP2. These findings will provide valuable information about the potential protein binding sites necessary for protein-ligand interactions which appear as attractive targets for the development of novel technologies and management strategies for insect pests"
Keywords:		"Animals Carrier Proteins/metabolism Female Insect Proteins/metabolism Ligands Molecular Docking Simulation *Moths/metabolism Pheromones/metabolism *Receptors, Odorant/metabolism *Sex Attractants/metabolism Zea mays/metabolism Athetis lepigone fluorescence;"
Notes:		"MedlineYang, Hui-Hui Xu, Ji-Wei Zhang, Xiao-Qing Huang, Jian-Rong Li, Lu-Lu Yao, Wei-Chen Zhao, Pan-Pan Zhang, Dong Liu, Jia-Yi Dewer, Youssef Zhu, Xiu-Yun Li, Xiao-Ming Zhang, Ya-Nan eng 2008085MC63/Natural Science Foundation of Anhui Province/ 202010373096 and S202013620012/Anhui Department of Education/ England 2022/02/25 Bull Entomol Res. 2022 Aug; 112(4):536-545. doi: 10.1017/S0007485321001127. Epub 2022 Feb 24"