| Title: | "A green leaf volatile, (Z)-3-hexenyl-acetate, mediates differential oviposition by Spodoptera frugiperda on maize and rice" |
| Author(s): | Wang J; Wei J; Yi T; Li YY; Xu T; Chen L; Xu H; |
| Address: | "National Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, China. School of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China. College of Forestry, Nanjing Forestry University, Nanjing, China. School of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China. chenli1@hbu.edu.cn. National Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, China. hhxu@scau.edu.cn" |
| DOI: | 10.1186/s12915-023-01642-x |
| ISSN/ISBN: | 1741-7007 (Electronic) 1741-7007 (Linking) |
| Abstract: | "BACKGROUND: Insects rely on chemosensory perception, mainly olfaction, for the location of mates, food sources, and oviposition sites. Plant-released volatile compounds guide herbivorous insects to search for and locate their host plants, further helping them to identify suitable positions for oviposition. The fall armyworm Spodoptera frugiperda (S. frugiperda) was found to invade China in 2019 and has since seriously threatened multiple crops, particularly maize and rice. However, the chemical and molecular mechanisms underlying oviposition preference in this pest are not fully understood. Here, the oviposition preference of S. frugiperda on maize and rice plants was investigated. RESULTS: GC-EAD and GC-MS/MS techniques were used to identify the antennally active volatiles from maize and rice plants. The attraction and oviposition stimulation of identified components to female adults were tested in both laboratory and field settings. The odorant receptors (ORs) on female antennae were expressed in Xenopus oocytes, and their functions evaluated by RNAi. Ten and eleven compounds of maize and rice plants, respectively, were identified to possess electrophysiological activity from headspace volatiles. Among these compounds, (Z)-3-hexenyl-acetate specifically presented in maize volatiles was found to play a critical role in attracting females and stimulating oviposition compared to rice volatiles. Among the cloned ORs on the antennae of both sexes, SfruOR23 with highly female-biased expression mediated the responses of females to (Z)-3-hexenyl-acetate. Knockdown of SfruOR23 using RNAi markedly reduced the electrophysiological response of female antennae and oviposition preference to the compound. CONCLUSIONS: (Z)-3-Hexenyl-acetate is a key volatile mediating the host and oviposition preference of S. frugiperda on maize. The olfactory receptor of (Z)-3-hexenyl-acetate was identified to be SfruOR23, which is mainly expressed in the antennae of S. frugiperda" |
| Keywords: | Animals Female Male Spodoptera *Zea mays *Oryza Oviposition Tandem Mass Spectrometry Acetates/metabolism Larva/physiology Olfactory receptor Oviposition preference Oviposition stimulation RNA interference Spodoptera frugiperda Volatile organic compounds; |
| Notes: | "MedlineWang, Jiali Wei, Jiaqi Yi, Ting Li, Ya-Ya Xu, Tian Chen, Li Xu, Hanhong eng Research Support, Non-U.S. Gov't England 2023/06/20 BMC Biol. 2023 Jun 19; 21(1):140. doi: 10.1186/s12915-023-01642-x" |
