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J Econ Entomol

Title:		Method for DNA Isolation From Sweetpotato Weevil (Coleoptera: Curculionidae) Collected in Pheromone-Baited Traps
Author(s):		Wadl PA; Williams LH; Harris-Shultz KR; McQuate GT;
Address:		"U.S. Vegetable Laboratory, United States Department of Agriculture, Agricultural Research Service, Charleston, SC. Crop Genetics and Breeding Research Unit, United States Department of Agriculture, Agricultural Research Service, Tifton, GA. Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center, United States Department of Agriculture, Agricultural Research Service, Hilo, HI"
Journal Title:		J Econ Entomol
Year:		2019
Volume:		112
Issue:		2
Page Number:		1001 - 1003
DOI:		10.1093/jee/toy390
ISSN/ISBN:		1938-291X (Electronic) 0022-0493 (Linking)
Abstract:		"This study provides a protocol for the isolation of high-quality DNA from sweetpotato weevils (Cylas formicarius elegantulus (Summers)) collected from pheromone-baited aerial funnel traps. This study was based on our discovery that a 2-wk collection interval of sweetpotato weevils from pheromone traps did not permit isolation of intact high-quality genomic DNA. To test the effect of collection methods, i.e., sample collection interval and preservation method, on quality of isolated DNA, we placed freshly killed male sweetpotato weevils into aerial funnel traps in the field and removed subsamples at several times thereafter. DNA yield from freshly isolated (day = 0) samples was significantly greater than samples preserved in 70% ethanol or at -20 degrees C, whereas there was no difference between 70% ethanol and -20 degrees C storage. Likewise, DNA yield from freshly isolated (day = 0) samples was significantly greater than for later sampling times. Quality assessment of genomic DNA through gel electrophoresis and polymerase chain reaction (PCR) indicated isolation of high molecular weight DNA for all samples collected at t
Keywords:		Animals *Coleoptera DNA *Ipomoea batatas Male Pheromones *Weevils Cylas formicarius elegantulus (Summers) DNA isolation aerial funnel trap;
Notes:		"MedlineWadl, Phillip A Williams, Livy H Harris-Shultz, Karen R McQuate, Grant T eng England 2018/12/28 J Econ Entomol. 2019 Mar 21; 112(2):1001-1003. doi: 10.1093/jee/toy390"