| Title: | Intergenerational environmental effects: functional signals in offspring transcriptomes and metabolomes after parental jasmonic acid treatment in apomictic dandelion |
| Author(s): | Verhoeven KJF; Verbon EH; van Gurp TP; Oplaat C; Ferreira de Carvalho J; Morse AM; Stahl M; Macel M; McIntyre LM; |
| Address: | "Terrestrial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10, Wageningen, the Netherlands. Plant-Microbe Interactions, Utrecht University, Padualaan 6, Utrecht, the Netherlands. Molecular Genetics and Microbiology, and the Genetics Institute, University of Florida, 2033 Mowry Road, Gainesville, FL, 32610, USA. Center for Plant Molecular Biology, Tubingen University, Auf der Morgenstelle 32, Tubingen, D-72076, Germany. Molecular Interaction Ecology, Department of Plant Science, Radboud University Nijmegen, PO Box 9010, Nijmegen, 6500 NL, the Netherlands" |
| ISSN/ISBN: | 1469-8137 (Electronic) 0028-646X (Print) 0028-646X (Linking) |
| Abstract: | "Parental environments can influence offspring traits. However, the magnitude of the impact of parental environments on offspring molecular phenotypes is poorly understood. Here, we test the direct effects and intergenerational effects of jasmonic acid (JA) treatment, which is involved in herbivory-induced defense signaling, on transcriptomes and metabolomes in apomictic common dandelion (Taraxacum officinale). In a full factorial crossed design with parental and offspring JA and control treatments, we performed leaf RNA-seq gene expression analysis, LC-MS metabolomics and total phenolics assays in offspring plants. Expression analysis, leveraged by a de novo assembled transcriptome, revealed an induced response to JA exposure that is consistent with known JA effects. The intergenerational effect of treatment was considerable: 307 of 858 detected JA-responsive transcripts were affected by parental JA treatment. In terms of the numbers of metabolites affected, the magnitude of the chemical response to parental JA exposure was c. 10% of the direct JA treatment response. Transcriptome and metabolome analyses both identified the phosphatidylinositol signaling pathway as a target of intergenerational JA effects. Our results highlight that parental environments can have substantial effects in offspring generations. Transcriptome and metabolome assays provide a basis for zooming in on the potential mechanisms of inherited JA effects" |
| Keywords: | "Apomixis/drug effects/*genetics Cluster Analysis Cyclopentanes/*pharmacology *Environment Gene Expression Regulation, Plant/drug effects Gene Ontology Metabolome/drug effects/*genetics Metabolomics Oxylipins/*pharmacology Phenols/metabolism Plant Leaves/d;Herbivory Induced defense;" |
| Notes: | "MedlineVerhoeven, Koen J F Verbon, Eline H van Gurp, Thomas P Oplaat, Carla Ferreira de Carvalho, Julie Morse, Alison M Stahl, Mark Macel, Mirka McIntyre, Lauren M eng U24 DK097209/DK/NIDDK NIH HHS/ Research Support, Non-U.S. Gov't England 2017/10/17 New Phytol. 2018 Jan; 217(2):871-882. doi: 10.1111/nph.14835. Epub 2017 Oct 16" |
