| Title: | Molecular cloning and characterization of a novel carotenoid cleavage dioxygenase 1 from Lycium chinense |
| Author(s): | Tian X; Ji J; Wang G; Jin C; Guan C; Wu G; |
| Address: | "School of Chemical Engineering and Technology, Tianjin University, Tianjin, People's Republic of China. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin, People's Republic of China. School of Environmental Science and Engineering, Tianjin University, Tianjin, People's Republic of China" |
| ISSN/ISBN: | 1470-8744 (Electronic) 0885-4513 (Linking) |
| Abstract: | "Carotenoids are key precursor for aroma compounds in plants. Although the fruit of Lycium chinense contains numerous carotenoids, the formation mechanism of aroma compounds in L. chinense is still poorly understood. In this study, a new carotenoid cleavage dioxygenase (termed LmCCD1) was identified from the leaves of L. chinense. Expression analysis by semiquantitative PCR reveals that LmCCD1 gene is expressed in different tissues of L. chinense, and dominant expression of LmCCD1 gene was found in leaves, flowers, and ripe fruits. In addition, the expression level of LmCCD1 in fruits is in accordance with the content of beta-ionone. Finally, recombinantly expressed LmCCD1 can cleave beta-carotene and lycopene to produce beta-ionone and pseudoionone in in vitro assays. These results indicate that LmCCD1 a novel carotenoids cleavage dioxygenase gene that may regulate the metabolic pathways responsible for aroma metabolite production (such as beta-ionone and pseudoionone) in L. chinense has been identified" |
| Keywords: | "Amino Acid Sequence Cloning, Molecular Dioxygenases/chemistry/*genetics/isolation & purification/*metabolism Gene Expression Regulation, Plant Lycium/*enzymology/*genetics/metabolism Molecular Sequence Data Volatile Organic Compounds/metabolism Lycium chi;" |
| Notes: | "MedlineTian, Xiaowei Ji, Jing Wang, Gang Jin, Chao Guan, Chunfeng Wu, Guangxia eng Research Support, Non-U.S. Gov't 2014/12/17 Biotechnol Appl Biochem. 2015 Nov-Dec; 62(6):772-9. doi: 10.1002/bab.1327. Epub 2015 Mar 30" |
