| Title: | The Importance of Protein Phosphorylation for Signaling and Metabolism in Response to Diel Light Cycling and Nutrient Availability in a Marine Diatom |
| Author(s): | Tan MH; Smith SR; Hixson KK; Tan J; McCarthy JK; Kustka AB; Allen AE; |
| Address: | "Scripps Institution of Oceanography, Integrative Oceanography Division, University of California, San Diego, La Jolla, CA 92037, USA. Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, CA 92037, USA. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354, USA. Department of Bioengineering, University of California, San Diego, La Jolla, CA 92037, USA. Department of Earth and Environmental Sciences, Rutgers University, Newark, NJ 07102, USA" |
| ISSN/ISBN: | 2079-7737 (Print) 2079-7737 (Electronic) 2079-7737 (Linking) |
| Abstract: | "Diatoms are major contributors to global primary production and their populations in the modern oceans are affected by availability of iron, nitrogen, phosphate, silica, and other trace metals, vitamins, and infochemicals. However, little is known about the role of phosphorylation in diatoms and its role in regulation and signaling. We report a total of 2759 phosphorylation sites on 1502 proteins detected in Phaeodactylum tricornutum. Conditionally phosphorylated peptides were detected at low iron (n = 108), during the diel cycle (n = 149), and due to nitrogen availability (n = 137). Through a multi-omic comparison of transcript, protein, phosphorylation, and protein homology, we identify numerous proteins and key cellular processes that are likely under control of phospho-regulation. We show that phosphorylation regulates: (1) carbon retrenchment and reallocation during growth under low iron, (2) carbon flux towards lipid biosynthesis after the lights turn on, (3) coordination of transcription and translation over the diel cycle and (4) in response to nitrogen depletion. We also uncover phosphorylation sites for proteins that play major roles in diatom Fe sensing and utilization, including flavodoxin and phytotransferrin (ISIP2A), as well as identify phospho-regulated stress proteins and kinases. These findings provide much needed insight into the roles of protein phosphorylation in diel cycling and nutrient sensing in diatoms" |
| Keywords: | diatom diel iron nitrogen stress phosphoproteome signaling; |
| Notes: | "PubMed-not-MEDLINETan, Maxine H Smith, Sarah R Hixson, Kim K Tan, Justin McCarthy, James K Kustka, Adam B Allen, Andrew E eng NSF-MCB-1024913/National Science Foundation/ NSF-MCB- 1818390/National Science Foundation/ NSF-OCE-0727997/National Science Foundation/ NSF-OCE-1756884/National Science Foundation/ DE-SC00006719/U.S. Department of Energy/ DE-SC0008593/U.S. Department of Energy/ GBMF3828/Gordon and Betty Moore Foundation/ Switzerland 2020/07/10 Biology (Basel). 2020 Jul 6; 9(7):155. doi: 10.3390/biology9070155" |
