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J Basic Microbiol

Title:		Genotypic intraspecies heterogeneity of Enterococcus italicus: data from dairy environments
Author(s):		Borgo F; Ferrario C; Ricci G; Fortina MG;
Address:		"Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione di Microbiologia Industriale, Universita degli Studi di Milano, Milan, Italy. francesca.borgo@unimi.it"
Journal Title:		J Basic Microbiol
Year:		2013
Volume:		20120514
Issue:		1
Page Number:		20 - 28
DOI:		10.1002/jobm.201100464
ISSN/ISBN:		1521-4028 (Electronic) 0233-111X (Linking)
Abstract:		"The diversity of a collection of 19 Enterococcus italicus strains isolated from different dairy sources was explored using a molecular polyphasic approach, comprising random amplification of polymorphic DNA (RAPD-PCR), repetitive element PCR (REP-PCR), plasmid profiling and ribotyping. The data obtained showed a high-level of biodiversity, not always correlated to the niche of isolation. Particularly, REP-PCR with primer BOXA1R and plasmid profiling allowed the best discrimination at strain level. Exploiting the genome shotgun sequence of the type strain of the species, available in public database, genes related to insertion sequences present on enterococcal Pathogenic Islands (ISEf1, IS905), determinants related to virulence factors (codifying for hemolysin and cell wall surface proteins), exogenously DNA (conjugal transfer protein, replication plasmid protein, pheromone shutdown protein, phage integrase/recombinase) and penicillin binding proteins system were detected. The presence of most of these genes seemed a common genetic trait in the Enterococcus genus, sur gene (cell wall surface protein) was only detected in strains of E. italicus. To our knowledge, this is the first time that specific primers, with the expection of the species-specific probe targeted to 16S rRNA gene, have been designed for this species"
Keywords:		"Animals Cattle Cheese/*microbiology Chickens DNA, Bacterial/analysis/chemistry/genetics Enterococcus/*classification/genetics/isolation & purification Feces/microbiology *Food Microbiology Genes, Bacterial Genetic Variation Genotype Humans Olea/microbiolo;"
Notes:		"MedlineBorgo, Francesca Ferrario, Chiara Ricci, Giovanni Fortina, Maria Grazia eng Research Support, Non-U.S. Gov't Germany 2012/05/15 J Basic Microbiol. 2013 Jan; 53(1):20-8. doi: 10.1002/jobm.201100464. Epub 2012 May 14"