| Title: | Bacteria detection based on the evolution of enzyme-generated volatile organic compounds: determination of Listeria monocytogenes in milk samples |
| Author(s): | Tait E; Perry JD; Stanforth SP; Dean JR; |
| Address: | "Department of Applied Sciences, Northumbria University, Ellison Building, Newcastle upon Tyne NE1 8ST, UK. Department of Microbiology, Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK. Department of Applied Sciences, Northumbria University, Ellison Building, Newcastle upon Tyne NE1 8ST, UK. Electronic address: John.Dean@northumbria.ac.uk" |
| DOI: | 10.1016/j.aca.2014.07.029 |
| ISSN/ISBN: | 1873-4324 (Electronic) 0003-2670 (Linking) |
| Abstract: | "The rapid detection of Listeria monocytogenes contamination in food is essential to prevent food-borne illness in humans. The aim of this study was to differentiate non-contaminated milk from milk contaminated with L. monocytogenes using enzyme substrates coupled with the analysis of volatile organic compounds (VOCs). The method is based on the activity of beta-glucosidase and hippuricase enzymes and the detection of a specific VOC i.e. 2-nitrophenol and 3-fluoroaniline, respectively. VOCs were extracted, separated and detected by headspace-solid phase microextraction coupled to gas chromatography-mass spectrometry (HS-SPME GC-MS). This approach required the inclusion of the selective agent's cycloheximide, nalidixic acid and acriflavine HCl in the growth medium to inhibit interfering bacteria. The VOCs were liberated by L. monocytogenes provided that samples contained at least 1-1.5x10(2) CFU ml(-1) of milk prior to overnight incubation. This approach shows potential for future development as a rapid method for the detection of L. monocytogenes contaminated milk" |
| Keywords: | Amidohydrolases/*metabolism Aniline Compounds/analysis/isolation & purification/metabolism Animals Fluorobenzenes/analysis/isolation & purification/metabolism Food Microbiology/*methods Gas Chromatography-Mass Spectrometry Listeria monocytogenes/enzymolog; |
| Notes: | "MedlineTait, Emma Perry, John D Stanforth, Stephen P Dean, John R eng Research Support, Non-U.S. Gov't Netherlands 2014/09/30 Anal Chim Acta. 2014 Oct 27; 848:80-87. doi: 10.1016/j.aca.2014.07.029. Epub 2014 Jul 24" |
