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Arch Microbiol

Title:		Isolation and composition of the constitutive agglutinins from haploid Saccharomyces cerevisiae cells
Author(s):		Sijmons PC; Nederbragt AJ; Klis FM; van den Ende H;
Address:		"Department of Plant Physiology, University of Amsterdam, The Netherlands"
Journal Title:		Arch Microbiol
Year:		1987
Volume:		148
Issue:		3
Page Number:		208 - 212
DOI:		10.1007/BF00414813
ISSN/ISBN:		0302-8933 (Print) 0302-8933 (Linking)
Abstract:		"Sex-specific agglutinins from the cell surface of haploid cells of Saccharomyces cerevisiae (X2180, mta and mt alpha) were purified and analysed. The constitutive agglutinin from mta cells was extractable with 3 mM dithiothreitol. It was shown to be a glycoprotein (3% mannose) with an apparent Mr of 43,000 based on gel filtration, but in SDS-PAGE it behaved as a much smaller molecule (Mr between 18,000 and 26,000). About one in three amino acids was a hydroxyamino acid. Its biological activity was resistant to boiling for 1 h, but sensitive to pronase. Intact mt alpha cells retained their agglutinability in the presence of dithiothreitol but limited trypsinizing released a biologically active agglutinin fragment. It had an apparent Mr of 320,000 (gel filtration). When analysed by SDS-PAGE, a single diffuse band with an apparent Mr of 225,000 was observed. The protein was 94% (w/w) mannose with a trace of N-acetyl glucosamine. Its biological activity was almost completely lost after boiling for 1 h. Both agglutinins behaved as monovalent molecules and specifically inhibited the biological activity of both noninduced and pheromone-induced cells. Pheromone treatment of mta cells resulted in an apparent 32-fold increase in agglutinin activity at the cell surface, whereas pheromone treatment of mt alpha cells only doubled the apparent agglutinin activity"
Keywords:		"Agglutinins/*analysis/isolation & purification Amino Acids/analysis Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Haploidy Saccharomyces cerevisiae/*analysis/physiology;"
Notes:		"MedlineSijmons, P C Nederbragt, A J Klis, F M Van den Ende, H eng Germany 1987/09/01 Arch Microbiol. 1987 Sep; 148(3):208-12. doi: 10.1007/BF00414813"