| Title: | 2-Aminoacetophenone as a potential breath biomarker for Pseudomonas aeruginosa in the cystic fibrosis lung |
| Author(s): | Scott-Thomas AJ; Syhre M; Pattemore PK; Epton M; Laing R; Pearson J; Chambers ST; |
| Address: | "Christchurch School of Medicine and Health Sciences, Department of Pathology, University of Otago, Christchurch, 8140, New Zealand. amy.scott-thomas@otago.ac.nz" |
| ISSN/ISBN: | 1471-2466 (Electronic) 1471-2466 (Linking) |
| Abstract: | "BACKGROUND: Pseudomonas aeruginosa infections are associated with progressive life threatening decline of lung function in cystic fibrosis sufferers. Growth of Ps. aeruginosa releases a 'grape-like' odour that has been identified as the microbial volatile organic compound 2-aminoacetophenone (2-AA). METHODS: We investigated 2-AA for its specificity to Ps. aeruginosa and its suitability as a potential breath biomarker of colonisation or infection by Solid Phase Micro Extraction and Gas Chromatography-Mass Spectrometry (GC/MS). RESULTS: Cultures of 20 clinical strains of Ps. aeruginosa but not other respiratory pathogens had high concentrations of 2-AA in the head space of in vitro cultures when analysed by GC/MS. 2-AA was stable for 6 hours in deactivated glass sampling bulbs but was not stable in Tedlar(R) bags. Optimisation of GC/MS allowed detection levels of 2-AA to low pico mol/mol range in breath. The 2-AA was detected in a significantly higher proportion of subjects colonised with Ps. aeruginosa 15/16 (93.7%) than both the healthy controls 5/17 (29%) (p < 0.0002) and CF patients not colonised with Ps. aeruginosa 4/13(30.7%) (p < 0.001). The sensitivity and specificity of the 2-AA breath test compared to isolation of Ps. aeruginosa in sputum and/or BALF was 93.8% (95% CI, 67-99) and 69.2% (95% CI, 38-89) respectively. The peak integration values for 2-AA analysis in the breath samples were significantly higher in Ps. aeruginosa colonised subjects (median 242, range 0-1243) than the healthy controls (median 0, range 0-161; p < 0.001) and CF subjects not colonised with Ps. aeruginosa (median 0, range 0-287; p < 0.003). CONCLUSIONS: Our results report 2-AA as a promising breath biomarker for the detection of Ps. aeruginosa infections in the cystic fibrosis lung" |
| Keywords: | Acetophenones/*analysis Adolescent Adult Biomarkers/analysis *Breath Tests/methods Child Cystic Fibrosis/complications/*microbiology Female Gas Chromatography-Mass Spectrometry Humans Lung/microbiology Male Middle Aged Pseudomonas Infections/complications; |
| Notes: | "MedlineScott-Thomas, Amy J Syhre, Mona Pattemore, Philip K Epton, Michael Laing, Richard Pearson, John Chambers, Stephen T eng Research Support, Non-U.S. Gov't England 2010/11/09 BMC Pulm Med. 2010 Nov 7; 10:56. doi: 10.1186/1471-2466-10-56" |
