| Title: | A novel class of peptide pheromone precursors in ascomycetous fungi |
| Author(s): | Schmoll M; Seibel C; Tisch D; Dorrer M; Kubicek CP; |
| Address: | "Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9/1665, Vienna 1060, Austria. monika.schmoll@tuwien.ac.at" |
| DOI: | 10.1111/j.1365-2958.2010.07295.x |
| ISSN/ISBN: | 1365-2958 (Electronic) 0950-382X (Print) 0950-382X (Linking) |
| Abstract: | "Recently, sexual development in the heterothallic ascomycete Trichoderma reesei (anamorph of Hypocrea jecorina) has been achieved and thus initiated attempts to elucidate regulation and determinants of this process. While the alpha-type pheromone of this fungus fits the consensus known from other fungi, the assumed a-type peptide pheromone precursor shows remarkably unusual characteristics: it comprises three copies of the motif (LI)GC(TS)VM thus constituting a CAAX domain at the C-terminus and two Kex2-protease sites. This structure shares characteristics of both a- and alpha-type peptide pheromone precursors. Presence of hybrid-type peptide pheromone precursor 1 (hpp1) is essential for male fertility, thus indicating its functionality as a peptide pheromone precursor, while its phosphorylation site is not relevant for this process. However, sexual development in a female fertile background is not perturbed in the absence of hpp1, which rules out a higher order function in this process. Open reading frames encoding proteins with similar characteristics to HPP1 were also found in Fusarium spp., of which Fusarium solani still retains a putative a-factor-like protein, but so far in no other fungal genome available. We therefore propose the novel class of h-type (hybrid) peptide pheromone precursors with H. jecorina HPP1 as the first member of this class" |
| Keywords: | "Computational Biology DNA, Fungal/genetics Fungal Proteins/*chemistry/classification/genetics Gene Deletion Gene Library Genetic Complementation Test Pheromones/*chemistry/classification/genetics Protein Precursors/*chemistry/classification Trichoderma/*c;" |
| Notes: | "MedlineSchmoll, Monika Seibel, Christian Tisch, Doris Dorrer, Marcel Kubicek, Christian P eng P 20004/FWF_/Austrian Science Fund FWF/Austria Research Support, Non-U.S. Gov't England 2010/08/26 Mol Microbiol. 2010 Sep; 77(6):1483-501. doi: 10.1111/j.1365-2958.2010.07295.x" |
