| Title: | Breathprinting Reveals Malaria-Associated Biomarkers and Mosquito Attractants |
| Author(s): | Schaber CL; Katta N; Bollinger LB; Mwale M; Mlotha-Mitole R; Trehan I; Raman B; Odom John AR; |
| Address: | "Department of Pediatrics, St. Louis, Missouri. Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri. Department of Biomedical Engineering, Washington University, St. Louis, Missouri. Lilongwe District Health Office, Malawi Ministry of Health, Blantyre, Malawi. Department of Pediatrics and Child Health, University of Malawi College of Medicine, Blantyre, Malawi. Department of Pediatrics, Kamuzu Central Hospital, Lilongwe, Malawi" |
| ISSN/ISBN: | 1537-6613 (Electronic) 0022-1899 (Print) 0022-1899 (Linking) |
| Abstract: | "Current evidence suggests that malarial infection could alter metabolites in the breath of patients, a phenomenon that could be exploited to create a breath-based diagnostic test. However, no study has explored this in a clinical setting. To investigate whether natural human malarial infection leads to a characteristic breath profile, we performed a field study in Malawi. Breath volatiles from children with and those without uncomplicated falciparum malaria were analyzed by thermal desorption-gas chromatography/mass spectrometry. Using an unbiased, correlation-based analysis, we found that children with malaria have a distinct shift in overall breath composition. Highly accurate classification of infection status was achieved with a suite of 6 compounds. In addition, we found that infection correlates with significantly higher breath levels of 2 mosquito-attractant terpenes, alpha-pinene and 3-carene. These findings attest to the viability of breath analysis for malaria diagnosis, identify candidate biomarkers, and identify plausible chemical mediators for increased mosquito attraction to patients infected with malaria parasites" |
| Keywords: | "Animals Anopheles/*pathogenicity Biomarkers/*chemistry/*metabolism Breath Tests/methods Child Child, Preschool Female Gas Chromatography-Mass Spectrometry/methods Humans Malaria, Falciparum/*diagnosis/*metabolism/parasitology Malawi Male Odorants/*analysi;" |
| Notes: | "MedlineSchaber, Chad L Katta, Nalin Bollinger, Lucy B Mwale, Mwawi Mlotha-Mitole, Rachel Trehan, Indi Raman, Barani Odom John, Audrey R eng R01 AI103280/AI/NIAID NIH HHS/ R21 AI123808/AI/NIAID NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2018/02/08 J Infect Dis. 2018 Apr 23; 217(10):1553-1560. doi: 10.1093/infdis/jiy072" |
