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BMC Plant Biol

Title:		Effects of vitro sucrose on quality components of tea plants (Camellia sinensis) based on transcriptomic and metabolic analysis
Author(s):		Qian Y; Zhang S; Yao S; Xia J; Li Y; Dai X; Wang W; Jiang X; Liu Y; Li M; Gao L; Xia T;
Address:		"State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 West Changjiang Rd, Hefei, 230036, Anhui, China. School of Biological and Food Engineering, Suzhou University, 49 Middle Bianhe Rd, Suzhou, 234000, Anhui, China. School of Life Science, Anhui Agricultural University, 130 West Changjiang Rd, Hefei, 230036, Anhui, China. School of Life Science, Anhui Agricultural University, 130 West Changjiang Rd, Hefei, 230036, Anhui, China. gaolp62@126.com. State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 West Changjiang Rd, Hefei, 230036, Anhui, China. xiatao62@126.com"
Journal Title:		BMC Plant Biol
Year:		2018
Volume:		20180618
Issue:		1
Page Number:		121 -
DOI:		10.1186/s12870-018-1335-0
ISSN/ISBN:		1471-2229 (Electronic) 1471-2229 (Linking)
Abstract:		"BACKGROUND: Tea plants [Camellia sinensis (L.) O. Kuntze] can produce one of the three most widely popular non-alcoholic beverages throughout the world. Polyphenols and volatiles are the main functional ingredients determining tea's quality and flavor; however, the biotic or abiotic factors affecting tea polyphenol biosynthesis are unclear. This paper focuses on the molecular mechanisms of sucrose on polyphenol biosynthesis and volatile composition variation in tea plants. RESULTS: Metabolic analysis showed that the total content of anthocyanins, catechins, and proanthocyanidins(PAs) increased with sucrose, and they accumulated most significantly after 14 days of treatment. Transcriptomic analysis revealed 8384 and 5571 differentially expressed genes in 2-day and 14-day sucrose-treated tea plants compared with control-treated plants. Most of the structural genes and transcription factors (TFs) involved in polyphenol biosynthesis were significantly up-regulated after 2d. Among these transcripts, the predicted genes encoding glutathione S-transferase (GST), ATP-binding cassette transporters (ABC transporters), and multidrug and toxic compound extrusion transporters (MATE transporters) appeared up regulated. Correspondingly, ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS) analysis revealed that the content of non-galloylated catechins and oligomeric PAs decreased in the upper-stem and increased in the lower-stem significantly, especially catechin (C), epicatechin (EC), and their oligomeric PAs. This result suggests that the related flavonoids were transported downward in the stem by transporters. GC/MS data implied that four types of volatile compounds, namely terpene derivatives, aromatic derivatives, lipid derivatives, and others, were accumulated differently after in vitro sucrose treatment. CONCLUSIONS: Our data demonstrated that sucrose regulates polyphenol biosynthesis in Camellia sinensis by altering the expression of transcription factor genes and pathway genes. Additionally, sucrose promotes the transport of polyphenols and changes the aroma composition in tea plant"
Keywords:		"Camellia sinensis/drug effects/genetics/*metabolism Gene Expression Profiling Gene Expression Regulation, Plant/drug effects Genes, Plant/genetics Metabolomics Polyphenols/metabolism Real-Time Polymerase Chain Reaction Sucrose/metabolism/*pharmacology Tra;"
Notes:		"MedlineQian, Yumei Zhang, Shuxiang Yao, Shengbo Xia, Jinxin Li, Yanzhi Dai, Xinlong Wang, Wenzhao Jiang, Xiaolan Liu, Yajun Li, Mingzhuo Gao, Liping Xia, Tao eng 31570694/Natural Science Foundation of China/ 31470689/Natural Science Foundation of China/ 31300577/Natural Science Foundation of China/ 20133418130001/Specialized Research Fund for the Doctoral Program of Higher Education/ 1408085QC51/Natural Science Foundation of Anhui Province, China/ KJ2017A441/Natural Science Foundation for Higher Education of Anhui Province/ 2016jb02/Natural Science Foundation of Suzhou University/ England 2018/06/20 BMC Plant Biol. 2018 Jun 18; 18(1):121. doi: 10.1186/s12870-018-1335-0"