| Title: | Multiple stir bar sorptive extraction combined with gas chromatography-mass spectrometry analysis for a tentative identification of bacterial volatile and/or semi-volatile metabolites |
| Author(s): | Berrou K; Dunyach-Remy C; Lavigne JP; Roig B; Cadiere A; |
| Address: | "Univ. Nimes, EA7352 CHROME, Rue du Dr G. Salan, 30021 Nimes Cedex 1, France. Institut National de la Sante et de la Recherche Medicale, U1047, Universite Montpellier, UFR de Medecine, 30908 Nimes, France; Department of Microbiology, CHU Nimes, Universite Montpellier, 30029 Nimes, France. Univ. Nimes, EA7352 CHROME, Rue du Dr G. Salan, 30021 Nimes Cedex 1, France. Electronic address: axelle.cadiere@unimes.fr" |
| DOI: | 10.1016/j.talanta.2018.11.042 |
| ISSN/ISBN: | 1873-3573 (Electronic) 0039-9140 (Linking) |
| Abstract: | "We propose a new approach combining the principles and advantages of stir bar sorptive extraction (SBSE) and headspace sorptive extraction (HSSE). Stir bars have so far never been used for the extraction of volatile/semi-volatile bacterial compounds. The effectiveness of two stir bars with polydimethylsiloxane (PDMS) or ethylene glycol/silicone (EGS) as sorbent was tested by performing sample extraction directly in gas chromatography (GC) vials containing bacterial cultures. Several combinations of desorption and extraction were tested at different growth times. When the extraction was carried out simultaneously with the EGS stir bar in headspace and the PDMS in the bacterial culture, the number of extracted compounds was significantly increased. Using both twisters increased the polarity range of the compounds found, and extraction at the end of the exponential phase of growth generated the best yields. This method was successfully applied to determine the production of 17 molecules by a strain of Staphylococcus aureus. In conclusion, this study paves the way for a new method for determining the volatile metabolite profile of bacteria, which can provide a promising innovative alternative in the identification of biomarkers" |
| Keywords: | "Adsorption Chemistry Techniques, Analytical/*instrumentation/methods Dimethylpolysiloxanes/chemistry Ethylene Glycol/chemistry Silicones/chemistry Staphylococcus aureus/*metabolism Volatile Organic Compounds/*analysis/chemistry/metabolism Bacterial volati;" |
| Notes: | "MedlineBerrou, Kevin Dunyach-Remy, Catherine Lavigne, Jean-Philippe Roig, Benoit Cadiere, Axelle eng Netherlands 2019/01/11 Talanta. 2019 Apr 1; 195:245-250. doi: 10.1016/j.talanta.2018.11.042. Epub 2018 Nov 15" |
