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Methods Mol Biol

Title:		Chemotropic Assay for Testing Fungal Response to Strigolactones and Strigolactone-Like Compounds
Author(s):		Pineda-Martos R; Di Pietro A; Turra D;
Address:		"School of Agricultural Engineering, Area of Agroforestry Engineering, University of Seville, Seville, Spain. Department of Genetics, University of Cordoba, Cordoba, Spain. Department of Agricultural Sciences, University of Naples Federico II, Naples, Italy. davturra@unina.it"
Journal Title:		Methods Mol Biol
Year:		2021
Volume:		2309
Issue:
Page Number:		105 - 111
DOI:		10.1007/978-1-0716-1429-7_9
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"Current knowledge on the mechanism of strigolactones (SLs) as signaling molecules during specific interactions in the rhizosphere is mainly related to the control of germination of parasitic weed seeds and hyphal branching of arbuscular mycorrhizal fungi. Thus, the role of plant secreted SLs in regulating the growth and development of root-colonizing fungi still remains controversial. Fusarium oxysporum can sense and respond to extracellular signals through oriented germ tube emergence and redirectioning of hyphal growth toward gradients of nutrients, sex pheromones, or plant root exudates. However, chemoattractant activity of SLs against microorganisms living in the soil has not been tested so far. Here we propose a quantitative chemotropic assay to understand if and how soil fungi could sense gradients of SLs and SLs-like sources. In the example case of F. oxysporum, hyphae of fungal representative mutants preferentially grow toward the synthetic SL analog GR24; and this chemotropic response requires conserved elements of the fungal invasive growth mitogen-activated protein kinase (MAPK) cascade"
Keywords:		"Fusarium/*drug effects/growth & development/metabolism Heterocyclic Compounds, 3-Ring/*pharmacology Lactones/*pharmacology Plant Growth Regulators/*pharmacology Plant Roots/*microbiology *Soil Microbiology Tropism/*drug effects Chemotropism Fungal signali;"
Notes:		"MedlinePineda-Martos, Rocio Di Pietro, Antonio Turra, David eng Research Support, Non-U.S. Gov't 2021/05/25 Methods Mol Biol. 2021; 2309:105-111. doi: 10.1007/978-1-0716-1429-7_9"