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J Comp Neurol

Title:		Loss of TRPC2 function in mice alters sex differences in brain regions regulating social behaviors
Author(s):		Pfau DR; Baribeau S; Brown F; Khetarpal N; Marc Breedlove S; Jordan CL;
Address:		"Neuroscience Program, Michigan State University, East Lansing, Michigan, USA"
Journal Title:		J Comp Neurol
Year:		2023
Volume:		20230727
Issue:		15
Page Number:		1550 - 1561
DOI:		10.1002/cne.25528
ISSN/ISBN:		1096-9861 (Electronic) 0021-9967 (Linking)
Abstract:		"The transient receptor potential cation channel 2 (TRPC2) conveys pheromonal information from the vomeronasal organ (VNO) to the brain. Both male and female mice lacking this gene show altered sex-typical behavior as adults. We asked whether TRPC2, highly expressed in the VNO, normally participates in the development of VNO-recipient brain regions controlling mounting and aggression, two behaviors affected by TRPC2 loss. We now report significant effects of TRPC2 loss in both the posterodorsal aspect of the medial amygdala (MePD) and ventromedial nucleus of the hypothalamus (VMH) of male and female mice. In the MePD, a sex difference in neuron number was eliminated by the TRPC2 knockout (KO), but the effect was complex, with fewer neurons in the right MePD of females, and fewer neurons in the left MePD of males. In contrast, MePD astrocytes were unaffected by the KO. In the ventrolateral (vl) aspect of the VMH, KO females were like wildtype (WT) females, but TRPC2 loss had a dramatic effect in males, with fewer neurons than WT males and a smaller VMHvl overall. We also discovered a glial sex difference in VMHvl of WTs, with females having more astrocytes than males. Interestingly, TRPC2 loss increased astrocyte number in males in this region. We conclude that TRPC2 normally participates in the sexual differentiation of the mouse MePD and VMHvl. These changes in two key VNO-recipient regions may underlie the effects of the TRPC2 KO on behavior"
Keywords:		Animals Female Male Mice Aggression/physiology Hypothalamus Neuroglia *Sex Characteristics *Social Behavior amygdala astrocyte laterality neuron sex difference stereology;neuroscience;
Notes:		"MedlinePfau, Daniel R Baribeau, Sarah Brown, Felix Khetarpal, Niki Marc Breedlove, S Jordan, Cynthia L eng R01 NS028421/NS/NINDS NIH HHS/ R01 NS045195/NS/NINDS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2023/07/27 J Comp Neurol. 2023 Oct; 531(15):1550-1561. doi: 10.1002/cne.25528. Epub 2023 Jul 27"