| Title: | Chemical structure and biological activity of a quorum sensing pheromone from Bacillus subtilis subsp. natto |
| Author(s): | Okada M; Nakamura Y; Hayashi S; Ozaki K; Usami S; |
| Address: | "Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: okadam@mol.f.u-tokyo.ac.jp. Graduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan" |
| DOI: | 10.1016/j.bmcl.2015.07.083 |
| ISSN/ISBN: | 1464-3405 (Electronic) 0960-894X (Linking) |
| Abstract: | "Bacillus subtilis subsp. natto secrets a peptide pheromone, named ComXnatto pheromone, as an inducer for biofilm formation containing poly-gamma-glutamic acid. Recently, the ComXnatto pheromone was identified to be a hexapeptide with an amino acid sequence of Lys-Trp-Pro-Pro-Ile-Glu, and the tryptophan residue was post-translationally modified with a farnesyl group. In order to determine the precise modification of the tryptophan residue, ComXnatto pheromone was synthesized using solid-phase peptide synthesis. Biological activity of the ComXnatto pheromone was then investigated. It was demonstrated that poly-gamma-glutamic acid production were accelerated by ComXnatto pheromone at more than 1 nM in natto" |
| Keywords: | Bacillus subtilis/chemistry/*metabolism Molecular Structure Pheromones/chemical synthesis/*chemistry/*metabolism Polyglutamic Acid/*analogs & derivatives/biosynthesis/chemistry *Quorum Sensing Bacillus subtilis Natto Posttranslational modification Quorum; |
| Notes: | "MedlineOkada, Masahiro Nakamura, Yuta Hayashi, Shunsuke Ozaki, Koki Usami, Syohei eng Research Support, Non-U.S. Gov't England 2015/08/16 Bioorg Med Chem Lett. 2015 Oct 1; 25(19):4293-6. doi: 10.1016/j.bmcl.2015.07.083. Epub 2015 Jul 30" |
