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Biochem Biophys Res Commun

Title:		Alpha-factor-directed synthesis of Bacillus stearothermophilus alpha-amylase in Saccharomyces cerevisiae
Author(s):		Nonato RV; Shishido K;
Address:		"Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Yokohama, Japan"
Journal Title:		Biochem Biophys Res Commun
Year:		1988
Volume:		152
Issue:		1
Page Number:		76 - 82
DOI:		10.1016/s0006-291x(88)80682-x
ISSN/ISBN:		0006-291X (Print) 0006-291X (Linking)
Abstract:		"Promoter and leader sequence of Bacillus stearothermophilus alpha-amylase gene were removed and the gene was joined in-frame to sequences encoding the leader region of Saccharomyces cerevisiae mating pheromone alpha-factor on plasmid p69A (a hybrid of pBR322 and S. cerevisiae 2-microns plasmid). S. cerevisiae cells were transformed with plasmids containing the hybrid genes, obtaining yeast transformants which exhibit a significant extra-cellular amylolytic activity in solid medium, but not in liquid medium. Levels of alpha-amylase activity in solid medium were found to depend on the mode of fusion of the alpha-amylase gene to the alpha-factor leader region"
Keywords:		"Amino Acid Sequence Base Sequence *Genes *Genes, Bacterial Genes, Fungal Geobacillus stearothermophilus/enzymology/*genetics Mating Factor Molecular Sequence Data Peptides/*genetics Pheromones/*genetics Plasmids Saccharomyces cerevisiae/*genetics alpha-Am;"
Notes:		"MedlineNonato, R V Shishido, K eng 1988/04/15 Biochem Biophys Res Commun. 1988 Apr 15; 152(1):76-82. doi: 10.1016/s0006-291x(88)80682-x"