| Title: | Bisphenol A effects on the growing mouse oocyte are influenced by diet |
| Author(s): | Muhlhauser A; Susiarjo M; Rubio C; Griswold J; Gorence G; Hassold T; Hunt PA; |
| Address: | "School of Molecular Biosciences, Washington State University, Pullman, Washington, USA" |
| DOI: | 10.1095/biolreprod.108.074815 |
| ISSN/ISBN: | 0006-3363 (Print) 1529-7268 (Electronic) 0006-3363 (Linking) |
| Abstract: | "Growing evidence suggests that exposure to bisphenol A (BPA) has the ability to disrupt several different stages of oocyte development. To date, most attention has focused on the effects of BPA on the periovulatory oocyte, and considerable variation is evident in the results of these studies. In our own laboratory, variation in the results of BPA studies conducted at different times appeared to correlate with changes in mill dates of animal feed. This observation, coupled with reports by others that dietary estrogens in feed are a confounding variable in studies of endocrine-disrupting chemicals, prompted us to evaluate the effect of diet on the results of BPA studies of the periovulatory oocyte. Genetically identical females were placed on a high- or low-phytoestrogen diet prior to mating. Their female offspring were exposed to BPA, oocytes collected, and meiotic spindle and chromosome characteristics compared between control and BPA-treated females. We observed significant diet-related variation in both the frequency of abnormalities in oocytes from untreated females and in the response to BPA. Our results demonstrate that the impact of BPA on meiosis depends, at least in part, on diet. We suggest that variation in the conclusions of recent BPA studies reflects differences in the diets used, as well as other methodological differences. Because meiotic disturbances are a feature of all studies to date, however, we conclude that low levels of BPA adversely affect the meiotic process" |
| Keywords: | "Aneuploidy Animal Feed/analysis Animals Benzhydryl Compounds *Diet Endocrine Disruptors/administration & dosage/toxicity Estrogens, Non-Steroidal/administration & dosage/*toxicity Female Isoflavones/administration & dosage Meiosis/drug effects/genetics Mi;" |
| Notes: | "MedlineMuhlhauser, Ailene Susiarjo, Martha Rubio, Carmen Griswold, Jodi Gorence, Galen Hassold, Terry Hunt, Patricia A eng R01 HD021341/HD/NICHD NIH HHS/ R01 ES013527/ES/NIEHS NIH HHS/ R37 HD021341/HD/NICHD NIH HHS/ ES013527/ES/NIEHS NIH HHS/ HD21341/HD/NICHD NIH HHS/ Research Support, N.I.H., Extramural 2009/01/24 Biol Reprod. 2009 May; 80(5):1066-71. doi: 10.1095/biolreprod.108.074815. Epub 2009 Jan 21" |
