| Title: | Green leaf volatile-burst in Arabidopsis is governed by galactolipid oxygenation by a lipoxygenase that is under control of calcium ion |
| Address: | "Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi, 753-8515, Japan. Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi, 753-8515, Japan. Electronic address: matsui@yamaguchi-u.ac.jp" |
| Journal Title: | Biochem Biophys Res Commun |
| DOI: | 10.1016/j.bbrc.2018.10.042 |
| ISSN/ISBN: | 1090-2104 (Electronic) 0006-291X (Linking) |
| Abstract: | "Plants form green leaf volatiles (GLVs) almost instantly after tissue disruption caused by damages, such as herbivore damage. This rapid formation of GLVs, namely GLV-burst, is an essential factor for the plants' GLV-dependent direct and indirect defenses. However, mechanism of GLV-burst remains unknown. We observed that the formation of monogalactosyldiacylglycerol hydroperoxides (MGDG-OOHs) by Arabidopsis lipoxygenase 2 (AtLOX2) governs GLV-burst in Arabidopsis. Addition of a Ca(2+) selective chelating reagent, BAPTA, during tissue disruption effectively suppressed the formation of MGDG-OOHs as well as GLV-burst. This suppression was relieved by the addition of Ca(2+). Therefore, we propose that Ca(2+)-dependent activation of AtLOX2 facilitates GLV-burst formation as observed in leukotriene formation, which is regulated by Ca(2+)-dependent activation of LOXs in animal cells" |
| Keywords: | Arabidopsis/*metabolism Arabidopsis Proteins/*metabolism Calcium/pharmacology Galactolipids/*biosynthesis/metabolism Hydrogen Peroxide Lipoxygenases/*metabolism Oxygen/metabolism Plant Immunity Plant Leaves/*metabolism Volatile Organic Compounds/*metaboli; |
| Notes: | "MedlineMochizuki, Satoshi Matsui, Kenji eng Research Support, Non-U.S. Gov't 2018/10/13 Biochem Biophys Res Commun. 2018 Nov 2; 505(3):939-944. doi: 10.1016/j.bbrc.2018.10.042. Epub 2018 Oct 9" |
