| Title: | Non-targeted screening and trimethylamine determination in Tilletia foetida-infected wheat using HS-SPME-GC-MS |
| Author(s): | Mei X; Wu X; Zhou F; Liu Y; Ji H; Li Y; Jiang D; Yang M; Xu J; Qiang Y; Wang C; Zhang Y; Zhang C; |
| Address: | "State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China. Key Laboratory of Biotoxin Analysis & Assessment for State Market Regulation, Nanjing Institute of Product Quality Inspection & Testing, Nanjing, China" |
| Journal Title: | Food Addit Contam Part A Chem Anal Control Expo Risk Assess |
| DOI: | 10.1080/19440049.2022.2154853 |
| ISSN/ISBN: | 1944-0057 (Electronic) 1944-0057 (Linking) |
| Abstract: | "Common bunt disease of wheat in China is caused mainly by Tilletia foetida. However, reliable approaches for determining disease-associated biochemical markers are rarely reported. Here, a headspace-solid-phase microextraction coupled with headspace GC-tandem mass spectrometry (HS-SPME-GC-MS) was used to screen volatile substances in infected wheat, and an optimal chemical marker was selected to establish analytical methods for disease determination. Non-targeted screening of 13 volatile compounds unique to diseased wheat allowed a metabolite with rotten fish-like smell, trimethylamine (TMA), to be selected as the inspection marker. Subsequently, two analytical methodologies, HS-SPME-GC-MS and headspace gas chromatography with flame ionization detection (HS-GC-FID), were established to determinate the TMA content in wheat. The linear relationship, recovery and reproducibility of the methods were validated. The limit of detection (LOD) was 0.02 microg/kg for the former method, 5000-fold lower than that for the latter. When analysing samples, HS-SPME-GC-MS showed excellent sensitivity and allowed for the determination of 0.05% infected kernels among whole wheat grains. Therefore, TMA determination using HS-SPME-GC-MS is an effective alternative method to detect wheat common bunt disease occurring at extremely low infection rate" |
| Keywords: | Animals *Solid Phase Microextraction/methods Triticum Gas Chromatography-Mass Spectrometry/methods Reproducibility of Results *Volatile Organic Compounds/analysis Tilletia foetida Wheat gas chromatograph-tandem mass spectrometry (GC-MS) solid-phase micro-; |
| Notes: | "MedlineMei, Xiuming Wu, Xiaoxiao Zhou, Fan Liu, Yanrong Ji, Hanxu Li, Yufeng Jiang, Diyao Yang, Miao Xu, Jingjing Qiang, Yuwei Wang, Can Zhang, Yu Zhang, Chi eng England 2022/12/16 Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2023 Feb; 40(2):181-192. doi: 10.1080/19440049.2022.2154853. Epub 2022 Dec 15" |
