| Title: | Investigation of the selenium metabolism in cancer cell lines |
| Author(s): | Lunoe K; Gabel-Jensen C; Sturup S; Andresen L; Skov S; Gammelgaard B; |
| Address: | "Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark" |
| ISSN/ISBN: | 1756-591X (Electronic) 1756-5901 (Linking) |
| Abstract: | "The aim of this work was to compare different selenium species for their ability to induce cell death in different cancer cell lines, while investigating the underlying chemistry by speciation analysis. A prostate cancer cell line (PC-3), a colon cancer cell line (HT-29) and a leukaemia cell line (Jurkat E6-1) were incubated with five selenium compounds representing inorganic as well as organic Se compounds in different oxidation states. Selenomethionine (SeMet), Se-methylselenocysteine (MeSeCys), methylseleninic acid (MeSeA), selenite and selenate in the concentration range 5-100 muM were incubated with cells for 24 h and the induction of cell death was measured using flow cytometry. The amounts of total selenium in cell medium, cell lysate and the insoluble fractions was determined by ICP-MS. Speciation analysis of cellular fractions was performed by reversed phase, anion exchange and size exclusion chromatography and ICP-MS detection. The selenium compounds exhibited large differences in their ability to induce cell death in the three cell lines and the susceptibilities of the cell lines were different. Full recovery of selenium in the cellular fractions was observed for all Se compounds except MeSeA. Speciation analysis showed that MeSeA was completely transformed during the incubations, while metabolic conversion of the other Se compounds was limited. Production of volatile dimethyl diselenide was observed for MeSeA and MeSeCys. MeSeA, MeSeCys and selenite showed noticeable protein binding. Correlations between cell death induction and the Se compounds transformations could not be demonstrated" |
| Keywords: | "Cell Line, Tumor HT29 Cells Humans Jurkat Cells Mass Spectrometry Neoplasms/chemistry/*metabolism Organoselenium Compounds/chemistry/*metabolism Selenium/chemistry/*metabolism Selenium Compounds/chemistry/*metabolism Subcellular Fractions/chemistry/metabo;" |
| Notes: | "MedlineLunoe, Kristoffer Gabel-Jensen, Charlotte Sturup, Stefan Andresen, Lars Skov, Soren Gammelgaard, Bente eng Research Support, Non-U.S. Gov't England 2010/12/17 Metallomics. 2011 Feb; 3(2):162-8. doi: 10.1039/c0mt00091d. Epub 2010 Dec 16" |
