| Title: | Anti-inflammation of isoliquiritigenin via the inhibition of NF-kappaB and MAPK in LPS-stimulated MAC-T cells |
| Author(s): | Li M; Lu G; Ma X; Wang R; Chen X; Yu Y; Jiang C; |
| Address: | "Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, No. 2 Tiansheng Road, Chongqing, 400715, China. Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, College of Animal Science and Technology, Southwest University, No. 2 Tiansheng Road, Chongqing, 400715, China. jcd2004@163.com" |
| DOI: | 10.1186/s12917-022-03414-1 |
| ISSN/ISBN: | 1746-6148 (Electronic) 1746-6148 (Linking) |
| Abstract: | "BACKGROUND: The application of plant extracts has received great interest for the treatment of bovine mastitis. Isoliquiritigenin (ISL) is a rich dietary flavonoid that has significant antioxidative, anti-inflammatory and anticancer activities. This study was conducted to explore the protective efficacy and related mechanism of ISL against lipopolysaccharide (LPS)-stimulated oxidation and inflammation in bovine mammary epithelial cells (MAC-T) by in vitro experiments. RESULTS: Real-time PCR and ELISA assays indicated that ISL treatment at 2.5, 5 and 10 mug/mL significantly reduced the mRNA and protein expression of the oxidative indicators cyclooxygenase-2 and inducible nitric oxide synthase (P < 0.01), and of the inflammatory cytokines interleukin-6 (P < 0.05), interleukin-1beta (P < 0.01) and tumor necrosis factor-alpha (P < 0.01) in LPS-stimulated MAC-T cells. Moreover, Western blotting and immunofluorescence tests indicated that the phosphorylation levels of nuclear factor kappa (NF-kappaB) p65 and the inhibitor of NF-kappaB were significantly decreased by ISL treatment, thus blocking the nuclear transfer of NF-kappaB p65. In addition, ISL attenuated the phosphorylation levels of p38, extracellular signal-regulated kinase and c-jun NH2 terminal kinase. CONCLUSIONS: Our data demonstrated that ISL downregulated the LPS-induced inflammatory response in MAC-T cells. The anti-inflammatory and antioxidative activity of ISL involves the NF-kappaB and MAPK cascades" |
| Keywords: | Animals Anti-Inflammatory Agents/pharmacology Cattle Chalcones Female *Lipopolysaccharides/toxicity *NF-kappa B/metabolism Nitric Oxide Synthase Type II/genetics T-Lymphocytes Isoliquiritigenin Mapk NF-kappaB anti-inflammation bovine mastitis; |
| Notes: | "MedlineLi, Manman Lu, Guicong Ma, Xiao Wang, Ruihong Chen, Xihong Yu, Yongxiong Jiang, Caode eng England 2022/08/20 BMC Vet Res. 2022 Aug 19; 18(1):320. doi: 10.1186/s12917-022-03414-1" |
