| Title: | Effect of production system and inhibitory potential of aroma volatiles on polyphenol oxidase and peroxidase activity in tomatoes |
| Author(s): | Lee JH; Kasote DM; Jayaprakasha GK; Avila CA; Crosby KM; Patil BS; |
| Address: | "Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX, USA. Department of Horticultural Sciences, Texas A&M Agrilife Research and Extension Center, Weslaco, TX, USA" |
| ISSN/ISBN: | 1097-0010 (Electronic) 0022-5142 (Linking) |
| Abstract: | "BACKGROUND: Polyphenol oxidase (PPO) and peroxidase (POD) are key enzymes associated with shelf life and defense responses. Thus, the activity of PPO and POD enzymes is usually assessed to check the quality of food samples and to understand the physiological responses of plants to different stresses. However, the outcomes of PPO and POD activity assessment studies are highly dependent on assay conditions. Hence, in this study, we initially optimized PPO and POD extraction and high-throughput 96-well plates-based enzymatic activity assessment methods to evaluate the inhibitory potential of tomato volatile compounds. Later, we explored the effects of net-house and open-field growing on the PPO and POD activity in tomato fruits of eight cultivars. RESULTS: We found 150 mM of catechol and pH 7.0 were the optimal conditions for the maximum activity for the PPO assay. Conversely, 24 mM guaiacol with 12 mM H(2) O(2) and pH 6.0 was the best condition for the POD assay. Thermal inactivation studies confirmed that tomato POD is more resistant to heat than PPO. We found that the production systems had a considerable genotype-specific impact on tomato PPO and POD activity. Moreover, amongst the volatiles that were studied, beta-damascenone and d-limonene showed 50% PPO inhibition at 40 and 80 mM, respectively. CONCLUSION: The results of this study can be used to improve the shelf-life of fresh tomato fruit and its products. The findings also underscore the significance of PPO and POD enzymes as physiological trait markers in the tomato crop and fruit quality improvement programs. (c) 2020 Society of Chemical Industry" |
| Keywords: | Catechol Oxidase/*chemistry Enzyme Assays Enzyme Stability Fruit/chemistry/enzymology Kinetics Solanum lycopersicum/*enzymology Odorants/analysis Peroxidase/*chemistry Volatile Organic Compounds/chemistry enzyme inhibition peroxidase polyphenol oxidase pr; |
| Notes: | "MedlineLee, Jisun Hj Kasote, Deepak M Jayaprakasha, Guddadarangavvanahally K Avila, Carlos A Crosby, Kevin M Patil, Bhimanagouda S eng NIFA-SCRI- 2017-51181-26834/National Institute of Food and Agriculture/ England 2020/07/06 J Sci Food Agric. 2021 Jan 15; 101(1):307-314. doi: 10.1002/jsfa.10644. Epub 2020 Aug 10" |
