| Title: | Crosstalk between cAMP and pheromone signalling pathways in Ustilago maydis |
| Author(s): | Kruger J; Loubradou G; Regenfelder E; Hartmann A; Kahmann R; |
| Address: | "Institut fur Genetik und Mikrobiologie der Universitat Munchen, Germany" |
| ISSN/ISBN: | 0026-8925 (Print) 0026-8925 (Linking) |
| Abstract: | "In the phytopathogenic basidiomycete Ustilago maydis mating and dikaryon formation are controlled by a pheromone/receptor system and the multiallelic b locus. Recently, a gene encoding a G protein alpha subunit, gpa3, was isolated and has subsequently been implicated in pheromone signal transduction. Mutants deleted for gpa3 are sterile and nonpathogenic, and exhibit a morphology that is similar to that of mutants with defects in the adenylate cyclase gene uac1. We have found that the sterility and mutant morphology of gpa3 deletion strains can be rescued by exogenous cAMP. In these mutants and in the corresponding wild-type strains, exogenous cAMP stimulates pheromone gene expression to a level comparable to that seen in the pheromone-stimulated state. In addition, we demonstrate that uac1 is epistatic to gpa3. We conclude that Gpa3 controls the cAMP signalling pathway in U. maydis and discuss how this pathway feeds into the pheromone response" |
| Keywords: | "Cyclic AMP/*metabolism/pharmacology *Fungal Proteins *GTP-Binding Protein alpha Subunits GTP-Binding Proteins/*genetics/metabolism Gene Expression Regulation, Fungal/drug effects Genetic Complementation Test *Heterotrimeric GTP-Binding Proteins Phenotype;" |
| Notes: | "MedlineKruger, J Loubradou, G Regenfelder, E Hartmann, A Kahmann, R eng Research Support, Non-U.S. Gov't Germany 1998/12/23 Mol Gen Genet. 1998 Nov; 260(2-3):193-8. doi: 10.1007/s004380050885" |
