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Nutrients

Title:		Insights into the Composition of a Co-Culture of 10 Probiotic Strains (OMNi BiOTiC((R)) AAD10) and Effects of Its Postbiotic Culture Supernatant
Author(s):		Kienesberger B; Obermuller B; Singer G; Arneitz C; Gasparella P; Klymiuk I; Horvath A; Stadlbauer V; Magnes C; Zugner E; Lopez-Garcia P; Trajanoski S; Miekisch W; Fuchs P; Till H; Castellani C;
Address:		"Department of Paediatric and Adolescent Surgery, Medical University of Graz, 8034 Graz, Austria. Department of Cell Biology, Histology and Embryology, Medical University of Graz, 8034 Graz, Austria. Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, 8034 Graz, Austria. Center of Biomarker Research (CBmed), 8034 Graz, Austria. Health-Institute for Biomedicine and Health Sciences, Joanneum Research, 8010 Graz, Austria. Core Facility Computational Bioanalytics, Medical University of Graz, 8034 Graz, Austria. Department of Anesthesiology and Intensive Care, Rostock University Medical Center, 18057 Rostock, Germany"
Journal Title:		Nutrients
Year:		2022
Volume:		20220311
Issue:		6
Page Number:		-
DOI:		10.3390/nu14061194
ISSN/ISBN:		2072-6643 (Electronic) 2072-6643 (Linking)
Abstract:		"BACKGROUND: We aimed to gain insights in a co-culture of 10 bacteria and their postbiotic supernatant. METHODS: Abundances and gene expression were monitored by shotgun analysis. The supernatant was characterized by liquid chromatography mass spectroscopy (LC-MS) and gas chromatography mass spectroscopy (GC-MS). Supernatant was harvested after 48 h (S48) and 196 h (S196). Susceptibility testing included nine bacteria and C. albicans. Bagg albino (BALBc) mice were fed with supernatant or culture medium. Fecal samples were obtained for 16S analysis. RESULTS: A time-dependent decrease of the relative abundances and gene expression of L. salivarius, L. paracasei, E. faecium and B. longum/lactis and an increase of L. plantarum were observed. Substances in LC-MS were predominantly allocated to groups amino acids/peptides/metabolites and nucleotides/metabolites, relating to gene expression. Fumaric, panthotenic, 9,3-methyl-2-oxovaleric, malic and aspartic acid, cytidine monophosphate, orotidine, phosphoserine, creatine, tryptophan correlated to culture time. Supernatant had no effect against anaerobic bacteria. S48 was reactive against S. epidermidis, L. monocytogenes, P. aeruginosae, E. faecium and C. albicans. S196 against S. epidermidis and Str. agalactiae. In vivo S48/S196 had no effect on alpha/beta diversity. Linear discriminant analysis effect size (LEfSe) and analysis of composition of microbiomes (ANCOM) revealed an increase of Anaeroplasma and Faecalibacterium prausnitzii. CONCLUSIONS: The postbiotic supernatant had positive antibacterial and antifungal effects in vitro and promoted the growth of distinct bacteria in vivo"
Keywords:		Animals Anti-Bacterial Agents/pharmacology Bacteria/genetics Candida albicans Coculture Techniques Mice *Probiotics/pharmacology culture microbiome postbiotic shotgun keyword susceptibility volatile organic compound;
Notes:		"MedlineKienesberger, Bernhard Obermuller, Beate Singer, Georg Arneitz, Christoph Gasparella, Paolo Klymiuk, Ingeborg Horvath, Angela Stadlbauer, Vanessa Magnes, Christoph Zugner, Elmar Lopez-Garcia, Pablo Trajanoski, Slave Miekisch, Wolfram Fuchs, Patricia Till, Holger Castellani, Christoph eng 867991/Austrian Research Promoting Agency/ n/a/Institut Allergosan, Graz, Austria/ Switzerland 2022/03/27 Nutrients. 2022 Mar 11; 14(6):1194. doi: 10.3390/nu14061194"