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J Insect Sci

Title:		Expression Profile and Functional Characterization Suggesting the Involvement of Three Chemosensory Proteins in Perception of Host Plant Volatiles in Chilo suppressalis (Lepidoptera: Pyralidae)
Author(s):		Khuhro SA; Yan Q; Liao H; Zhu GH; Sun JB; Dong SL;
Address:		"Department of Entomology, College of Plant Protection, Nanjing Agricultural University/ Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing, China"
Journal Title:		J Insect Sci
Year:		2018
Volume:		20180901
Issue:		5
Page Number:		-
DOI:		10.1093/jisesa/iey088
ISSN/ISBN:		1536-2442 (Electronic) 1536-2442 (Linking)
Abstract:		"The high sensitivity of the olfactory system is essential for feeding and oviposition in moth insects, and some chemosensory proteins (CSPs) are thought to play roles in this system by binding and carrying hydrophobic odorants across the aqueous sensillar lymph. In this study, to identify the olfactory CSPs from a repertoire of 21 CSP members in the notorious rice pest Chilo suppressalis (Walker) (Lepidoptera: Pyralidae), tissue expression patterns were firstly examined by quantitative real-time polymerase chain reaction (qPCR). It showed that CSP2 was antennae specific and seven more CSPs (CSP1, 3, 4, 6, 15, 16, and 17) were antennae biased in expression, suggesting their olfactory roles; while other CSPs were multiple-tissue expressed and non-antennae biased, suggesting other functions for these genes. To further determine the ligand binding specificity, three putative olfactory genes (CSP1-3) were expressed in Escherichia coli cells, and binding affinity of these three recombinant CSP proteins were measured for 35 plant volatiles by the ligand binding assays. CSP1 and CSP2 exhibited high binding affinities (Ki
Keywords:		Animals Arthropod Antennae/*physiology Female Gene Expression Profiling Insect Proteins/*metabolism Male Moths/genetics/*physiology *Olfactory Perception Real-Time Polymerase Chain Reaction Sex Factors Volatile Organic Compounds/*metabolism;
Notes:		"MedlineKhuhro, Sajjad Ali Yan, Qi Liao, Hui Zhu, Guan-Heng Sun, Jia-Bin Dong, Shuang-Lin eng 2018/09/28 J Insect Sci. 2018 Sep 1; 18(5):6. doi: 10.1093/jisesa/iey088"