| Title: | The G-alpha Gpa1 directs septin localization in the mating projection of Saccharomyces cerevisiae through its Ubiquitination Domain and Endocytic Machinery |
| Author(s): | Johnson CP; Hart A; Jarvis KF; Latario SG; Shrestha S; Leclerc N; Khalil A; Kelley JB; |
| DOI: | 10.1101/2023.06.16.545321 |
| Abstract: | "The yeast mating response uses a G-protein coupled receptor (GPCR), Ste2, to detect mating pheromone and initiate mating projection morphogenesis. The septin cytoskeleton plays a key role in the formation of the mating projection, forming structures at the base of the projection. Desensitization of the Galpha, Gpa1, by the Regulator of G-protein Signaling (RGS), Sst2, is required for proper septin organization and morphogenesis. In cells where the Galpha is hyperactive, septins are mislocalized to the site of polarity, and the cells are unable to track a pheromone gradient. We set out to identify the proteins that mediate Galpha control of septins during the Saccharomyces cerevisiae mating response by making mutations to rescue septin localization in cells expressing the hyperactive Galpha mutant gpa1 (G302S) . We found that single deletions of the septin chaperone Gic1, the Cdc42 GAP Bem3, and the epsins Ent1 and Ent2 rescued the polar cap accumulation of septins in the hyperactive Galpha. We created an agent based model of vesicle trafficking that predicts how changes in endocytic cargo licensing alters localization of endocytosis that mirrors the septin localization we see experimentally. We hypothesized that hyperactive Galpha may increase the rate of endocytosis of a pheromone responsive cargo, thereby altering where septins are localized. Both the GPCR and the Galpha are known to be internalized by clathrin-mediated endocytosis during the pheromone response. Deletion of the GPCR C-terminus to block internalization partially rescued septin organization. However, deletion of the Gpa1 ubiquitination domain required for its endocytosis completely abrogated septin accumulation at the polarity site. Our data support a model where the location of endocytosis serves as a spatial mark for septin structure assembly and that desensitization of the Galpha delays its endocytosis sufficiently that septins are placed peripheral to the site of Cdc42 polarity" |
| Notes: | "PubMed-not-MEDLINEJohnson, Cory P Hart, Andrew Jarvis, Katherine F Latario, Sarah G Shrestha, Sudati Leclerc, Nicholas Khalil, Andre Kelley, Joshua B eng R15 GM140409/GM/NIGMS NIH HHS/ Preprint 2023/07/03 bioRxiv. 2023 Jun 16:2023.06.16.545321. doi: 10.1101/2023.06.16.545321. Preprint" |
