« Previous Abstract"Environmental application of nanotechnology: air, soil, and water"    Next AbstractBreathomics for the clinician: the use of volatile organic compounds in respiratory diseases »

BMJ Open

Title:		Assessment of breath volatile organic compounds in acute cardiorespiratory breathlessness: a protocol describing a prospective real-world observational study
Author(s):		Ibrahim W; Wilde M; Cordell R; Salman D; Ruszkiewicz D; Bryant L; Richardson M; Free RC; Zhao B; Yousuf A; White C; Russell R; Jones S; Patel B; Awal A; Phillips R; Fowkes G; McNally T; Foxon C; Bhatt H; Peltrini R; Singapuri A; Hargadon B; Suzuki T; Ng LL; Gaillard E; Beardsmore C; Ryanna K; Pandya H; Coates T; Monks PS; Greening N; Brightling CE; Thomas P; Siddiqui S;
Address:		"Department of Respiratory Sciences, College of Life Sciences, University of Leicester, Leicester, UK. University Hospitals of Leicester NHS Trust, Leicester, UK. Department of Chemistry, University of Leicester, Leicester, UK. Department of Chemistry, Loughborough University, Loughborough, UK. NIHR Leicester Clinical Research Facility, Leicester, UK. Paediatric Clinical Investigation Centre, Leicester, UK. Department of Cardiovascular Sciences, Cardiovascular Research Centre, University of Leicester, Leicester, UK. Leicester NIHR Biomedical Research Centre (Cardiovascular Theme), Leicester, UK. Discovery Medicine, Respiratory Therapeutic Area, GlaxoSmithKline PLC, Stevenage, UK. Department of Cardiovascular Sciences, University of Leicester, Leicester, UK"
Journal Title:		BMJ Open
Year:		2019
Volume:		20190308
Issue:		3
Page Number:		e025486 -
DOI:		10.1136/bmjopen-2018-025486
ISSN/ISBN:		2044-6055 (Electronic) 2044-6055 (Linking)
Abstract:		"INTRODUCTION: Patients presenting with acute undifferentiated breathlessness are commonly encountered in admissions units across the UK. Existing blood biomarkers have clinical utility in distinguishing patients with single organ pathologies but have poor discriminatory power in multifactorial presentations. Evaluation of volatile organic compounds (VOCs) in exhaled breath offers the potential to develop biomarkers of disease states that underpin acute cardiorespiratory breathlessness, owing to their proximity to the cardiorespiratory system. To date, there has been no systematic evaluation of VOC in acute cardiorespiratory breathlessness. The proposed study will seek to use both offline and online VOC technologies to evaluate the predictive value of VOC in identifying common conditions that present with acute cardiorespiratory breathlessness. METHODS AND ANALYSIS: A prospective real-world observational study carried out across three acute admissions units within Leicestershire. Participants with self-reported acute breathlessness, with a confirmed primary diagnosis of either acute heart failure, community-acquired pneumonia and acute exacerbation of asthma or chronic obstructive pulmonary disease will be recruited within 24 hours of admission. Additionally, school-age children admitted with severe asthma will be evaluated. All participants will undergo breath sampling on admission and on recovery following discharge. A range of online technologies including: proton transfer reaction mass spectrometry, gas chromatography ion mobility spectrometry, atmospheric pressure chemical ionisation-mass spectrometry and offline technologies including gas chromatography mass spectroscopy and comprehensive two-dimensional gas chromatography-mass spectrometry will be used for VOC discovery and replication. For offline technologies, a standardised CE-marked breath sampling device (ReCIVA) will be used. All recruited participants will be characterised using existing blood biomarkers including C reactive protein, brain-derived natriuretic peptide, troponin-I and blood eosinophil levels and further evaluated using a range of standardised questionnaires, lung function testing, sputum cell counts and other diagnostic tests pertinent to acute disease. ETHICS AND DISSEMINATION: The National Research Ethics Service Committee East Midlands has approved the study protocol (REC number: 16/LO/1747). Integrated Research Approval System (IRAS) 198921. Findings will be presented at academic conferences and published in peer-reviewed scientific journals. Dissemination will be facilitated via a partnership with the East Midlands Academic Health Sciences Network and via interaction with all UK-funded Medical Research Council and Engineering and Physical Sciences Research Council molecular pathology nodes. TRIAL REGISTRATION NUMBER: NCT03672994"
Keywords:		"Acute Disease Adult Breath Tests Cardiovascular Diseases/*diagnosis Data Collection Diagnosis, Differential Dyspnea/*diagnosis Exhalation Gas Chromatography-Mass Spectrometry Humans Multicenter Studies as Topic/*methods Observational Studies as Topic/*met;"
Notes:		"MedlineIbrahim, Wadah Wilde, Michael Cordell, Rebecca Salman, Dahlia Ruszkiewicz, Dorota Bryant, Luke Richardson, Matthew Free, Robert C Zhao, Bo Yousuf, Ahmed White, Christobelle Russell, Richard Jones, Sheila Patel, Bharti Awal, Asia Phillips, Rachael Fowkes, Graham McNally, Teresa Foxon, Clare Bhatt, Hetan Peltrini, Rosa Singapuri, Amisha Hargadon, Beverley Suzuki, Toru Ng, Leong L Gaillard, Erol Beardsmore, Caroline Ryanna, Kimuli Pandya, Hitesh Coates, Tim Monks, Paul S Greening, Neil Brightling, Christopher E Thomas, Paul Siddiqui, Salman eng PDF-2017-10-052/DH_/Department of Health/United Kingdom MR/N005880/1/MRC_/Medical Research Council/United Kingdom Clinical Trial Protocol Research Support, Non-U.S. Gov't England 2019/03/11 BMJ Open. 2019 Mar 8; 9(3):e025486. doi: 10.1136/bmjopen-2018-025486"