| Title: | Identification of biosynthetic intermediates for the mating hormone alpha2 of the plant pathogen Phytophthora |
| Author(s): | Ariyoshi S; Imazu Y; Ohguri R; Katsuta R; Yajima A; Shibata T; Ojika M; |
| Address: | "Graduate School of Bioagricultural Sciences, Nagoya University, Tokai National Higher Education and Research System, Chikusa-ku, Nagoya, Japan. Graduate School of Agriculture, Tokyo Agricultural University, Setagaya-ku, Tokyo, Japan. Department of Chemistry for Life Sciences and Agriculture, Faculty of Life Sciences, Tokyo Agricultural University, Setagaya-ku, Tokyo, Japan" |
| Journal Title: | Biosci Biotechnol Biochem |
| ISSN/ISBN: | 1347-6947 (Electronic) 0916-8451 (Linking) |
| Abstract: | "The heterothallic group of the plant pathogen Phytophthora can sexually reproduce between the cross-compatible mating types A1 and A2. The mating hormone alpha2, produced by A2 mating type and utilized to promote the sexual reproduction of the partner A1 type, is known to be biosynthesized from phytol. In this study, we identified 2 biosynthetic intermediates, 11- and 16-hydroxyphytols (1 and 2), for alpha2 by administering the synthetic intermediates to an A2-type strain to produce alpha2 and by administering phytol to A2 strains to detect the intermediates in the mycelia. The results suggest that alpha2 is biosynthesized by possibly 2 cytochrome P450 oxygenases via 2 hydroxyphytol intermediates (1 and 2) in A2 hyphae and secreted outside" |
| Keywords: | Mating Factor/*biosynthesis/chemistry Phytophthora/*metabolism Spectrum Analysis/methods Stereoisomerism Phytophthora biosynthesis mating hormone plant pathogen sexual reproduction; |
| Notes: | "MedlineAriyoshi, Suguru Imazu, Yusuke Ohguri, Ryuji Katsuta, Ryo Yajima, Arata Shibata, Takahiro Ojika, Makoto eng 26252015/Japan Society for the Promotion of Science/ England 2021/06/01 Biosci Biotechnol Biochem. 2021 Jul 23; 85(8):1802-1808. doi: 10.1093/bbb/zbab098" |
